PerkinElmer

Alpha试剂

OF

Virtually any target can be quickly and easily measured using Alpha technology, an improved alternative to quantitative ELISA assays and ELISA reagents. Such targets include enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous assay with no wash requirement and no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the homogenous assay right for you:

  • AlphaLISA® – when running large numbers of ELISA assays from the same sample or working with a difficult matrix, serum or plasma
  • AlphaLISA® SureFire® Ultra and AlphaScreen® SureFire® – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein-protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf quantitative ELISA assay, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

 

Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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"Alpha试剂" 1-50951 产品与服务

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AlphaLISA immunoassay kits

CD40 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human CD40 enables the quantitative determination of human cluster of differentiation 40 protein in serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

FASL (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human FASL enables the quantitative determination of human FAS ligand in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Alpha donor antibody coated

Anti-DNP Alpha Donor Beads

Alpha Donor beads conjugated to anti-DNP antibody. These beads can be used to capture DNP-labeled proteins, peptides, and small molecules.
AlphaLISA immunoassay kits

NGAL (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for NGAL is designed for the quantitative determination of Neutrophil Gelatinase Associated Lipocalin (NGAL) also known as lipocalin 2 in cell culture media and human serum using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with mouse NGAL, rat NGAL, bovine NGAL and chicken NGAL.
AlphaLisa Analyte 2

CD70 (human), lyophilized (AlphaLISA)

Recombinant human CD70 standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human CD70 detection kit, but can also be ordered separately.

IL-10 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interleukin-10 (IL-10) Detection Kit is designed for detection and quantitation of human IL-10 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Fc-fragment-conjugated Alpha Donor beads

Fc-fragment (human) conjugated Alpha Donor Beads

Alpha Donor beads conjugated to human IgG Fc fragment. These beads can be used to capture Fc binding proteins and receptors.
SureFire principle

AlphaScreen SureFire Akt 1/2 (p-Ser473) Assay Kit

The AlphaScreen® SureFire® p-Akt 1/2 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt 1/2 (phosphorylated on Ser473) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

CCL4/MIP-1ß (human) AlphaLISA Detection Kit

The AlphaLISA® Human C-C Motif Chemokine 4 / Macrophage Inflammatory Protein-1beta (CCL4 / MIP-1ß) Detection Kit is designed for detection and quantitation of human CCL4/MIP-1ß in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
AlphaLISA immunoassay kits

CXCL13 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human CXCL13 enables the quantitative determination of human C-X-C Motif Chemokine Ligand 13 in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
PerkinElmer

Alpha SureFire Ultra Lysis Buffer (5X)

Additional buffer for Alpha SureFire Ultra assays.

G-CSF (human) AlphaLISA Detection Kit

The AlphaLISA® Human Granulocyte Colony-Stimulating Factor (G-CSF) Detection Kit is designed for detection and quantitation of human G-CSF in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

Anti-Chicken IgY AlphaLISA® Acceptor beads

AlphaLISA® Acceptor beads conjugated to anti-chicken IgY antibody. The antibody is a rabbit polyclonal antibody that is Fc-specific. These beads can be used to capture chicken IgY antibodies for AlphaLISA no-wash assays.

FCGR1/CD64 AlphaLISA Binding Kit

This kit is designed for the detection of binding between FCGR1 (CD64) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding.
Alpha donor antibody coated

Anti-GFP Alpha Donor Beads

Alpha Donor beads conjugated to anti-GFP antibody. The antibody is a mouse monoclonal antibody that recognizes wildtype and recombinant GFP (rGFP), and cross-reacts with eGFP. These beads can be used to capture GFP-tagged proteins for AlphaScreen, AlphaLISA, and AlphaPlex no-wash assays.

Progesterone AlphaLISA Detection Kit

The AlphaLISA® Progesterone Detection Kit is designed for detection and quantitation of progesterone in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
SureFire principle

AlphaScreen SureFire ALK (p-Tyr1604) Assay Kit

The AlphaScreen® SureFire® p-ALK (Tyr1604) assay is a sandwich immunoassay for quantitative detection of phospho-ALK (phosphorylated on Tyr1604) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

CCL3/MIP-1α (human) AlphaLISA Detection Kit

The AlphaLISA® Human C-C Motif Chemokine 3 / Macrophage Inflammatory Protein-1alpha (CCL3 / MIP-1α) Detection Kit is designed for detection and quantitation of human CCL3/MIP-1α in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
AlphaLISA immunoassay kits

IL-28B (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-28B enables the detection and quantitation of human Interleukin-28B in human serum, plasma, lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human IL28A, IL29 or Mouse IL28B. Cross reactivity with other species has not been tested.
PerkinElmer

BTK (human), liquid (AlphaLISA)

Recombinant human BTK standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human BTK detection kit, but can also be ordered separately.
AlphaLisa Analyte 2

PD-L2 (human), lyophilized (AlphaLISA)

Recombinant human PD-L2 standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human PD-L2 detection kit, but can also be ordered separately.
AlphaLISA SureFire Ultra kits

Phospho-AMPKα 1/2 (Thr172) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra p-AMPKα 1/2 (Thr172) assay is a sandwich immunoassay for quantitative detection of phospho-AMPKα (phosphorylated on Thr172) in cellular lysates using Alpha Technology.
AlphaLISA Cellular Epigenetics kits

AlphaLISA dimethylated-Histone H3 Lysine 36 (H3K36me2) Cellular Detection Kit

This kit is designed for the detection of di-methylated Histone H3 Lysine 36 (H3K36me2) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

CHO-P AlphaLISA Detection Kit

AlphaLISA® no-wash immunoassay kit for detection of Chinese Hamster Ovary Host Cell Proteins (CHO HCP) in buffered solution or cell culture medium.

AlphaLISA Anti-di-methyl-Histone H3 Lysine 36 (H3K36me2) Acceptor Beads (for peptide substrate only)

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 di-methylated at lysine 36 (H3K36me2). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.
AlphaPlex-545-antibody-bead2

Anti-human IgG AlphaPlex 545 Acceptor Beads

AlphaPlex™-545 (Terbium) Acceptor beads conjugated to anti-human IgG antibody, for use in Alpha no-wash multiplexing assays.

FCGR2B/CD32b AlphaLISA Binding Kit

This kit is designed for the detection of binding between FCGR2B (CD32b) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapeutics by using competitive binding.
alphaimmunoassay1

Growth Hormone (human) AlphaLISA Detection Kit

The AlphaLISA® Human Growth Hormone Detection Kit is designed for detection and quantitation of human growth hormone in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
AlphaLISA immunoassay kits

IFN-γ (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA Human IFNγ Biotin-Free Detection Kit is designed for the quantitative detection of human IFNγ in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
AlphaScreen cAMP assay principle

AlphaScreen cAMP Detection Kit

AlphaScreen no-wash assay kit for quantitative detection of cAMP.

IgM (human) AlphaLISA Detection Kit

The AlphaLISA® Human IgM Protein Detection Kit is designed for detection and quantitation of human IgM in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-EGF Receptor (Tyr1068) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-EGF Receptor (Tyr1068) in a multiplexed assay.
AlphaLISA immunoassay kits

Amyloid ß 1-x (human) AlphaLISA Detection Kit

The AlphaLISA® Human Amyloid beta 1-x (Aß 1-x) Detection Kit is designed for detection and quantitation of human Aß 1-x in cerebrospinal fluid (CSF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
AlphaLisa Analyte 2

Human IgG, lyophilized (AlphaLISA)

Human IgG, lyophilized, 3 µg. Human IgG is included in the AlphaLISA® human IgG kits, but can also be ordered separately as a standalone item. One vial contains an amount of human IgG sufficient for performing 10 standard curves.

IL-15 (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA® Human IL-15 Biotin-Free Detection Kit is designed for the quantitative detection of human Interleukin 15 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit is compatible with high-biotin culture media and other sample types that contain high levels biotin.
AlphaLISA immunoassay kits

Periostin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human periostin enables the quantitative determination of human periostin in buffer, cell culture media, and serum using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

CD276 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human CD276 enables the quantitative determination of human Cluster of Differentiation 276 (CD276, B7-H3) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

CD70 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human CD70 enables the quantitative determination of human CD70 in buffer, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

GLP-1 (7-36) (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human GLP-1 enables the detection and quantitation of human GLP-1 (7-36 amide) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA SureFire Ultra kits

Phospho-p38 MAPK (Thr180/Tyr182) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra p-p38 MAPK (Thr180/Tyr182) assay is a sandwich immunoassay for quantitative detection of phospho-p38 MAPK (phosphorylated on Tyr180/182) in cellular lysates using Alpha Technology.

IgE (mouse) isotyping AlphaLISA Detection Kit

This AlphaLISA® immunoassay kit enables the isotyping of mouse IgE antibodies in different sample matrices, including cell culture media. The IgE subtype plays a role in allergic reactions, parasitic infections and hypersensitivity reactions.
AlphaLISA immunoassay kits

Leptin (human) AlphaLISA Detection Kit

The AlphaLISA® Human Leptin Detection Kit is designed for detection and quantitation of human leptin in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. Due to the presence of leptin binding proteins in serum or other matrices, an acid-dissociation step might be required before performing the AlphaLISA assay.
AlphaLisa Analyte 2

CD28 (human), lyophilized (AlphaLISA)

Human CD28 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human CD28 Detection Kit, but can also be ordered separately.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-EGFR (Tyr1068) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-EGFR (Tyr1068) assay is a sandwich immunoassay for quantitative detection of phospho-EGF Receptor (phosphorylated on Tyr1068) in cellular lysates using Alpha Technology.
Alpha_Donor_bead_1
AlphaLISA immunoassay kits

AST (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human AST enables the quantitative determination of human aspartate transaminase (AST) in buffered solution, cell culture media, and serum using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

TNFα (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine TNFα enables the detection and quantitation of porcine TNFα in porcine serum, plasma, and, cell lysyate, cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minimal cross-reactivity with human and bovine TNFα and no cross reactivity towards porcine IFNγ.
SureFire principle

AlphaScreen SureFire cJun (p-Ser73) Assay Kit

The AlphaScreen® SureFire® p-c-Jun (Ser73) assay is a sandwich immunoassay for quantitative detection of phospho-c-Jun (phosphorylated on Ser73) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Alpha donor antibody coated

Anti-rat IgG Alpha Donor Beads

Alpha Donor beads conjugated to anti-rat IgG antibody. This bead can be used to capture rat IgG antibodies for Alpha no-wash protein detection, antibody detection, antibody binding, and other applications.

Anti-Mouse IgG3 (isotyping) AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to a anti-mouse IgG3. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.

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