PerkinElmer

Alpha SureFire Kits

OF

Alpha SureFire® 技术是快速,灵敏和定量检测细胞蛋白质磷酸化水平的试剂平台。SureFire 技术可以完成细胞培养及分析物检测“一体化”模式,极大的简化了耗时的分离及洗涤步骤。SureFire基于微珠的三明治免疫测定法将Alpha供体和受体珠一起引入,并引起级联的化学反应以产生放大的信号来测定内源性蛋白质磷酸化水平。其也可以与转染的细胞一起使用,并且能够以基于细胞的形式精确研究各种信号传导途径,受体和激酶靶标。

Alpha SureFire® 系列产品包括, AlphaScreen SureFire® 和 AlphaLISA SureFire® Ultra,后者作为新一代蛋白质磷酸化检测技术,将 CaptSure 技术与 AlphaLISA微珠完美结合,免受杂抗体的干扰,既可应用于治疗性大分子抗体药筛选也可用于传统小分子药物筛选。 Alpha SureFire 试剂盒可以提供:

  • 作为Western-Blotting的一体化替代方案
  • 可灵活使用不同细胞系,干细胞和原代细胞
  • 具有成本效益和灵活性 - 单个微孔板可适用于任何目标的分析
  • 卓越的灵敏度和动态范围
  • 简单实现自动化
  • 非放射性试剂且无任何有害化学物质和,无需特殊后处理要求

Alpha SureFire Multiplex assay principle
Alpha SureFire Multiplex assay principle

Alpha SureFire® Ultra Multiplex

The Alpha SureFire® Ultra™ Multiplex assay kits allow the rapid, sensitive, and quantitative detection of phosphoproteins from cells, combined with the measurement of the total amount of the AKT, ERK or cofilin protein or combined with the measurement of a second phosphoprotein. This Alpha Multiplex measurement is carried out in the same assay plate well from a single sample of cell lysate, and is achieved by the use of two types of Alpha Acceptor beads that emit at distinct wavelengths (545nm and 615nm). The two distinct Alpha Acceptor beads report their binding to a distinct antigen through their association with specific assay antibodies, as indicated below.

Alpha SureFire Ultra Multiplex assays are divided into two categories (1) Multiplex SureFire Ultra (MPSU) kits and (2) Terbium SureFire Ultra (TBSU) kits. The MPSU kits are complete kits for phospho and total detection of the same target (ERK or AKT), while the TBSU kits measure a single phosphoprotein target and are intended to be combined with a standard AlphaLISA SureFire Ultra kit (ALSU) to assess the second target. The TBSU kits allow for a pick-and-pack combination of any two phosphoprotein targets in the assay lists, providing measurement of two separate targets with ultimate flexibility of selection.

  • High sensitivity and dynamic range – detect low levels of protein phosphorylation, with internal normalization for total protein content
  • No interference from spiked antibodies – uses CaptSure™ technology instead of protein A
  • 2- incubation protocol that can be run in 2 hrs for faster results
  • Convenience of choosing HTS-compatible version or 96-well High Volume (HV) format for low-throughput applications - for all the targets
  • More than 1000 combinations of either phospho X phospho target or phospho x total targets are available
AlphaLISA SureFire assay principle
AlphaLISA SureFire assay principle

AlphaLISA® SureFire® Ultra

The next-generation technology for detecting protein phosphorylation, AlphaLISA SureFire Ultra is a highly sensitive assay for screening therapeutic antibodies and small molecules on cultured cells or tissue lysates. AlphaLISA SureFire Ultra combines CaptSure™ technology with AlphaLISA Acceptor beads for a more efficient, faster, and simpler assay protocol.

  • High sensitivity and dynamic range – detect low levels of protein phosphorylation
  • No interference from spiked antibodies – uses CaptSure™ technology instead of protein A
  • Almost 2-fold increase in 384-well plates working volumes for easier pipetting, compared to AlphaScreen SureFire
  • 1- or 2- incubation protocol that can be run in 2 hrs for faster results
  • Convenience of choosing HTS-compatible version or 96-well High Volume (HV) format for low-throughput applications - for all the targets
  • Ideal tool when working with samples such as tissue lysates - AlphaLISA Acceptor beads have better spectral characteristics with regard to hemoglobin light quenching

With CaptSure™ technology, there is no interference from spiked antibodies, and with a lower background signal, you have better sensitivity and dynamic range, resulting in more – and more accurate – information from all your samples.

AlphaScreen SureFire assay principle
AlphaScreen SureFire assay principle

AlphaScreen® SureFire®

 

The traditional platform for a wide variety of applications, AlphaScreen® SureFire® is a no-wash assay for the detection of phosphorylated proteins in cells. The AlphaScreen SureFire kits use an immunosandwich assay based on a combination of protein A and streptavidin for the capture of the sandwich antibodies.

  • Simple protocol that can be run in 4 hrs
  • Great sensitivity and dynamic range
  • Convenience of choosing HTS-compatible version or 96-well High Volume (HV) format for low-throughput applications - for some targets only
  • Currently the widest choice of phospho-targets in our catalogue
  • 产品与服务

  • 资源,活动及更多信息

缩小结果
应用
  • Protein Analysis & Detection (120)
  • Kinase (117)
  • Multiplexing (33)
检测点(200-50,000)
  • 50000 (127)
  • 500 (126)
  • 10000 (125)
  • 100 (63)
检测目标
  • Akt1/2/3 (6)
  • p70 S6K (6)
  • ERK1/2 (6)
  • STAT1 (5)
  • p38 MAPK (5)
  • S6RP (4)
  • MEK1 (4)
  • VEGF2 Receptor (4)
  • 4EBP1 (4)
  • STAT3 (4)
  • Cofilin (4)
  • Akt1 (4)
  • STAT5 (4)
  • IKKα (4)
  • NFκB (4)
  • Topo IIα (3)
  • GAPDH (3)
  • SYK (3)
  • INSR (3)
  • mTOR (3)
  • eIF4E (3)
  • ALK (3)
  • SMAD3 (3)
  • GSK3β (3)
  • EGFR (3)
  • SLP-76 (3)
  • SMAD1 (2)
  • IκBα (2)
  • BAD (2)
  • AKT1/2/3 (2)
  • CREB (2)
  • c-Met (2)
  • STAT6 (2)
  • c-Jun (2)
  • eIF2α (2)
  • ErbB2 (2)
  • STAT4 (2)
  • JNK1/2/3 (2)
  • IGFR (2)
  • Caspase-9 (1)
  • p53 (1)
  • ATF2 (1)
  • AMPKα (1)
  • JNK1/3 (1)
  • SMAD2 (1)
  • GSK3α (1)
  • β-Catenin (1)
  • Elk1 (1)
  • Akt1/2 (1)
  • TrkB (1)
  • Chk-2 (1)
  • Hsp27 (1)
  • Chk-1 (1)
  • Acetyl CoA carboxylase (1)
  • MKK3/6 (1)
  • TrkA (1)
  • PDK1 (1)
检测目标类
  • Phosphoprotein (117)
  • Protein (25)
自动化兼容
  • Yes (140)
检测方法
  • Alpha (144)
实验类型
  • In vitro (81)
格式
  • Microplates (95)
分子修饰
  • Phosphorylation (54)
产品品牌名称
  • AlphaScreen SureFire (62)
  • AlphaLISA SureFire Ultra (47)
  • Alpha SureFire Ultra Multiplex (33)
  • AlphaPlex (2)
目标种
  • Human (87)
治疗领域
  • Autophagy (1)

"Alpha SureFire Kits" 1-50144 产品与服务

每页
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total GAPDH Assay Kit

The AlphaLISA® SureFire® Ultra™ Total GAPDH assay is a sandwich immunoassay for quantitative detection of GAPDH in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for cell number.
SureFire principle

AlphaScreen SureFire S6RP (p-Ser240;Ser244) Assay Kit

The AlphaScreen® SureFire® p-Ribosomal Protein S6 (p-Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-RP S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total GAPDH (Human/Mouse) Assay Kit

The AlphaLISA® SureFire® Ultra Mouse/Human GAPDH assay is a sandwich immunoassay for quantitative detection of human or mouse GAPDH in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization assay.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-SLP-76 (Ser376) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SLP-76 (Ser376) in a multiplexed assay.
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr421/Ser424) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr421/Ser424) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr421/Ser424) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-MEK1 (Ser218/222) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-MEK1 (Ser218/222) in a multiplexed assay.
AlphaLISA SureFire Ultra kits

Phospho-AMPKα 1/2 (Thr172) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra p-AMPKα 1/2 (Thr172) assay is a sandwich immunoassay for quantitative detection of phospho-AMPKα (phosphorylated on Thr172) in cellular lysates using Alpha Technology.
SureFire principle

AlphaScreen SureFire Phospho-Caspase 9 (Ser196) Kit

The AlphaScreen® SureFire® p-Caspase 9 (Ser196) assay is a sandwich immunoassay for quantitative detection of phospho-Caspase 9 (phosphorylated on Ser196) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
SureFire principle

AlphaScreen SureFire JNK 1-3 (p-Thr183;Tyr185) Assay Kit

The AlphaScreen® SureFire® p-JNK1/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-SMAD1 (Ser463/465) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SMAD1 (Ser463/465) in a multiplexed assay.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total mTOR Assay Kit

The AlphaLISA® SureFire® Ultra Total mTOR assay is a sandwich immunoassay for quantitative detection of mTOR (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
AlphaLISA SureFire Ultra kits

Phospho-mTOR (Ser2448) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-mTOR (Ser2448) assay is a sandwich immunoassay for quantitative detection of phospho-mTOR (phosphorylated on Ser2448) in cellular lysates using Alpha no-wash technology.
alphascreen surefire total

AlphaScreen SureFire Total IkB alpha Assay Kit

The AlphaScreen® SureFire® total p70 S6K assay is a sandwich immunoassay for quantitative detection of p70 S6K (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total SLP-76 Assay Kit

The AlphaLISA® SureFire® Ultra Total SLP-76 assay is a sandwich immunoassay for quantitative detection of SLP-76 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
SureFire principle

AlphaScreen SureFire Phospho-GSK 3a (Ser21) Kit

The AlphaScreen® SureFire® p-GSK 3α (Ser21) assay is a sandwich immunoassay for quantitative detection of phospho-GSK3α (phosphorylated on Ser21) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-mTOR (Ser2448) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-mTOR (Ser2448) in a multiplexed assay.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-Insulin Receptor β (Tyr1150/1151) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-Insulin Receptor β (Tyr1150/1151) assay is a sandwich immunoassay for quantitative detection of phospho-INSR (phosphorylated on Tyr1150/1151) in cellular lysates using Alpha Technology.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-AKT1/2/3 (Thr308) Assay Kit

The AlphaLISA® SureFire® Ultra p-AKT1/2/3 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Thr308) in cellular lysates using Alpha Technology.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-JNK 1/2/3 (Thr183/Tyr185) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-JNK 1/2/3 (Thr183/Tyr185) in a multiplexed assay.
SureFire principle

AlphaScreen SureFire EIF4E (p-Ser209) Assay Kit

The AlphaScreen® SureFire® p-eIF4E (Ser209) assay is a sandwich immunoassay for quantitative detection of phospho-eIF4E (phosphorylated on Ser209) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
SureFire principle

AlphaScreen SureFire IKKb (p-Ser177/Ser181) Assay Kit

The AlphaScreen® SureFire® p-IKKα (Ser177/181) assay is a sandwich immunoassay for quantitative detection of phospho-IKKα (phosphorylated on Ser177/181) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr229) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr229) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr229) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA SureFire Ultra kits

Phospho-JNK1/2/3 (Thr183/Tyr185) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-JNK1/2/3 (Thr183/Tyr185) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha no-wash technology.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-p38 MAPK (Thr180/Tyr182) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-p38 MAPK (Thr180/Tyr182) in a multiplexed assay.
AlphaLISA SureFire Ultra kits

Phospho-c-Met (Tyr1234/1235) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra p-c-Met (Tyr1234/1235) assay is a sandwich immunoassay for quantitative detection of phospho-c-Met (phosphorylated on Tyr1234/1235) in cellular lysates using Alpha Technology.
SureFire principle

AlphaScreen SureFire Akt 1/2 (p-Ser473) Assay Kit

The AlphaScreen® SureFire® p-Akt 1/2 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt 1/2 (phosphorylated on Ser473) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
SureFire principle

Phospho-ERK (Thr202/Tyr204) Monoclonal AlphaScreen SureFire Assay kit

The AlphaScreen® SureFire® p-ERK1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK1/2 (phosphorylated on Thr202/Tyr204) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA SureFire Ultra kits

Phospho-STAT1 (Tyr701) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-STAT1 (Tyr701) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Tyr701) in cellular lysates using Alpha Technology.
SureFire principle

AlphaScreen SureFire Phospho-Topo IIa (Thr1343) Assay Kit

The AlphaScreen® SureFire® p-Topoisomerase II-α (p-Thr1343) assay is a sandwich immunoassay for quantitative detection of phospho-Topoisomerase II-α (phosphorylated on Thr1343) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
SureFire principle

AlphaScreen SureFire CHK2 (p-Thr68) Assay Kit 

The AlphaScreen® SureFire® p-CHK2 (Thr68) assay is a sandwich immunoassay for quantitative detection of phospho-CHK2 (phosphorylated on Thr68 in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-STAT3 (Tyr705) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT3 (Tyr705) in a multiplexed assay.
SureFire principle

AlphaScreen SureFire Phospho-p53 (Ser392) Assay Kit

The AlphaScreen® SureFire® p-p53 (p-Ser392) assay is a sandwich immunoassay for quantitative detection of phospho-p53 (phosphorylated on Ser392) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total SYK Assay Kit

The AlphaLISA® SureFire® Ultra Total SYK assay is a sandwich immunoassay for quantitative detection of SYK (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
AlphaLISA SureFire Ultra kits

Phospho-MEK1 (Ser218/222) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-MEK1 (Ser218/222) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-MEK1 (phosphorylated on Ser218/222) in cellular lysates using Alpha no-wash technology.
Alpha SureFire Ultra Multiplex kits

Alpha SureFire® Ultra™ HV Multiplex p-Akt 1/2/3 (S473) (Eu) + Total Akt1 (Tb) Kit

The Alpha SureFire® Ultra™ HV Multiplex p-Akt 1/2/3 (Ser473) + Total Akt 1 assay kit is used to measure both the phosphorylation (Ser473) and total levels of endogenous Akt in cellular lysates. The kit measures phosphorylation on Ser473 of Akt 1/2/3, and total levels of Akt 1 only. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure Akt (Ser473) activation by either recombinant or endogenous receptors, and can be applied to primary cells.
alphascreen surefire total

AlphaScreen SureFire Total Akt1 research kit

The AlphaScreen® SureFire® Akt 1 (total) assay is a sandwich immunoassay for quantitative detection of Akt 1 in cellular lysates using Alpha Technology. The assay is an ideal system for normalizing Akt levels for experiments measuring changes in Akt phosphorylation. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
SureFire principle

AlphaScreen SureFire Phospho-Topo IIa (Ser1106) Assay Kit

The AlphaScreen® SureFire® p-Topoisomerase II-α (p-Ser1106) assay is a sandwich immunoassay for quantitative detection of phospho-Topoisomerase II-α (phosphorylated on Ser1106) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
SureFire principle

AlphaScreen SureFire Phospho-ATF2 (Thr71) Assay Kit

The AlphaScreen® SureFire® p-ATF2 (Thr71) assay is a sandwich immunoassay for quantitative detection of phospho-ATF2 (phosphorylated on Thr71) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
SureFire principle

AlphaScreen SureFire Phospho-GSK 3b (Ser9) Kit

The AlphaScreen® SureFire® p-GSK 3β (Ser9) assay is a sandwich immunoassay for quantitative detection of phospho-GSK3β (phosphorylated on Ser9) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SMAD3 (Ser423/425) Assay Kit

The AlphaLISA® SureFire® Ultra p-SMAD3 (Ser423/Ser425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/Ser425) in cellular lysates using Alpha Technology.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-eIF4E (Ser209) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-eIF4E (Ser209) in a multiplexed assay.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-STAT5 (Tyr694/699) Assay Kit

The AlphaLISA® SureFire® Ultra p-STAT5 (Tyr694/699) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-STAT5 (phosphorylated on Tyr694/699) in cellular lysates using Alpha Technology. This kit replaces ALSU-PST5-A-HV. ALSU-PST5-A-HV is no longer available as the antibody(ies) have been discontinued by the supplier. A new antibody pair has been selected and validated to make this new version, with a similar or slightly improved performance over the initial version. Comparative data are included in the manuals of the new kits.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total Akt1 Assay Kit

The AlphaLISA® SureFire® Ultra™ Total Akt1 assay is a sandwich immunoassay for quantitative detection of Akt1 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
SureFire principle

AlphaScreen SureFire Phospho-c-Met (Tyr1234/1235) Assay Kit 

The AlphaScreen® SureFire® p-c-Met (Tyr1234/1235) assay is a sandwich immunoassay for quantitative detection of phospho-c-Met (phosphorylated on Tyr1234/1235) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
SureFire principle

AlphaScreen SureFire S6RP (p-Ser235;Ser236) Assay Kit

The AlphaScreen® SureFire® p-Ribosomal Protein S6 (p-Ser235/236) assay is a sandwich immunoassay for quantitative detection of phospho-RP S6 (phosphorylated on Ser235/236) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately, in addition to the AlphaScreen SureFire kit.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-STAT5 (Tyr694/699) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT5 (Tyr694/699) in a multiplexed assay.
AlphaLISA SureFire Ultra kits

Phospho-Cofilin (Ser3) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra p-Cofilin (Ser3) assay is a sandwich immunoassay for quantitative detection of phospho-cofilin (phosphorylated on Ser3) in cellular lysates using Alpha Technology.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-Insulin Receptor β (Tyr1150/1151) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-Insulin Receptor β (Tyr1150/1151) in a multiplexed assay.
AlphaLISA SureFire Ultra kits

Total MEK1 AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ Total MEK1 assay is a sandwich immunoassay for quantitative detection of MEK1 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
AlphaLISA SureFire Ultra kits

Total Cofilin AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra Total Cofilin assay is a sandwich immunoassay for quantitative detection of cofilin (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

"Alpha SureFire Kits" 1-50144 产品与服务

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