PerkinElmer

CRISPR Workflows

YOUR PATHWAY TOWARD CRISPR WORKFLOWS

Functional genomic screens enable the modulation of hundreds or thousands of genes in a single experiment to identify genetic pathways, cellular processes, novel therapeutic targets, and to genetically profile existing or potential therapeutics.

PerkinElmer’s wide range of CRISPR solutions are designed to support you in every step of your CRISPR workflow. From CRISPR and RNAi libraries for high-throughput functional genomic screening to automated image platforms and cellular assays, we offer all the tools and technologies to help you edit with confidence and maximize your chance of success.

A Better Understanding Starts Here

Explore by Application 

CRISPR has exploded within the scientific research community and contributed to many notable breakthroughs. With a ride range of important applications, the technology has become pivotal for research and beyond. 

arrow Disease Models
  • Use CRISPR knockout, CRISPR activation, and CRISPR interference to understand specific gene contribution to a disease
  • Use gene knock-in to insert mutations mimicking disease or wild-type biology for better validated targets or compound identification
arrow Pathway Analysis
  • Understand the function of genes and how they contribute to biological processes and diseases
arrow Target Identification
  • Identify relevant targets or hits from phenotypic screening
  • Assess the contribution of each gene in the genome to compound activity in a full genome loss-of-function screen
arrow Target Validation
  • Verify targets in an arrayed secondary screen (smaller set of genes) and eliminate false positives
  • Investigate gene knockout or modification in a variety of cell types to determine if a gene target provides the same phenotype in multiple cell types
arrow Cell & Gene Therapy
  • Reverse disease-causing mutations
  • Edit CAR-T cells to attack cancer cells

The Various Types of CRISPR

Nimbus

A CRISPR WORKFLOW

Gene Editing Reagents

Selecting the appropriate format of CRISPR reagents is the first critical step before starting a screen. We offer a variety of formats, so that regardless of the scientific question, we can support your experimental needs.

Cas9 nuclease

Cas9 nuclease reagents are available as Cas9 protein, mRNA, plasmid, or lentiviral Cas9. For optimization and enrichment, versions co-expressing a fluorescent tag are available.

Edit-R CRISPR Guide RNA

Edit-R ™ CRISPR guide RNA are available in pools or as individual reagents, as predefined CRISPR knockout libraries, or a library can be designed using the cherry-pick library tool.

Homology-directed Repair Knock-in templates

HDR relies on the presence of a donor template with sufficient homology to the regions flanking the cut site. The homology arms’ length and donor type will depend on the modification being made. Our templates make it easy to customize a donor oligo.

Transfection and ancillary reagents

Successful introduction of gRNA and Cas9 protein into the cell is key to any gene-editing experiment to ensure efficiency. Our range of transfection reagents provide efficient and reliable delivery of CRISPR gRNA with minimal cellular toxicity.

Sample Prep & Automation

These types of assays are often labor intensive and require many steps for re-suspending the CRISPR reagents, cell transfer, and treatment addition or staining. To accelerate your assay, we offer a full range of liquid handling, integrated laboratory automation, and cell counters that together with our specially designed microplates, improve your workflow and provide consistent and quality results. 

For research use only. Not for use in diagnostic procedures.

JANUS Liquid Handler Workstations

The JANUS® G3 automated liquid handling workstations offer flexible automated sample preparation solutions to meet your specific application needs. The JANUS G3 portfolio includes both dedicated, application-specific workstations and personalized automated solutions. The JANUS G3 workstation can be ...

Integrated Laboratory Automation

From cellular screening and imaging applications to high-throughput screening and genomics-based applications, custom explorer™ G3 integrated workstations provide innovative application-focused laboratory automation solutions which simplify microplate handling, liquid handling, and detection. With c ...

Microplates

Choosing the right microplate is a critical, often overlooked, part of an assay. The right microplate helps provides valuable data, whereas the wrong microplate can lead to missed or inaccurate data leading to missed project timelines and ultimately higher costs. New technologies for drug developmen ...

Cell Counters

Nexcelom offers a broad range of slide or plate-based automated cell counters featuring brightfield and fluorescent read-out capabilities.

Detection

Once it's time to acquire an image, options for read-outs can range from simple phenotypic or viability assays to multiparametric high-content analysis. Our line of sensitive yet powerful microplate readers, live cell imagers, and high-content analysis systems, plus innovative reagents enable you to accelerate your workflow and reduce costs. 

Cellular Imaging Reagents

PerkinElmer's PhenoVue™ suite of cellular imaging reagents – including cell painting kits, organelle and cell compartment stains, fluorescent-labeled secondary antibodies, and cell function reagents – complements our proven high-content screening instruments and image analysis software. Cellular ima ...

High Content Screening Instruments

High-Content Analysis (HCA) or High-Content Screening (HCS) combines high-throughput automated imaging and analysis to extract quantitative multi-parametric data at the single-cell level. Originally developed as a complementary technology to traditional biochemical high-throughput screening (HTS) in ...

Microplate Readers

As many of you know, microplate readers are instruments used to detect biological, chemical, biochemical or physical events from samples in a microplate. This is where it all starts for your basic research, assay development and therapeutic research. A powerful and reliable plate reader is what you ...

Drug Discovery Reagents

Drug discovery starts with an understanding of the basics of diseases and disease pathways. Throughout the process of unraveling the complexity of disease pathways, identifying and characterizing targets, high throughput screening, and lead optimization PerkinElmer has the assays you can count on. P ...

Celigo Imaging Cytometer

The Celigo image cytometry system provides high-throughput, whole-well imaging and quantitative data through image analysis in bright field and up to four fluorescent channels, for a wide variety of cell-based assays.

Fluorescent Reagents

Nexcelom Bioscience offers a wide range of fluorescent reagents and kits for cell counting and cell-based assays. Our reagents are optimized to work with Cellometer and Celigo imaging systems as well as other fluorescence-based instruments.

Analysis

Once your gene-editing experiment is complete, you’re faced  with readouts and large amounts of data that need to be analyzed. Our next generation informatics solutions help you to contextually analyze all that data, so you have more physiologically relevant results to make more informed decisions.

Signals Image Artist

Signals Image Artist™ is our next generation image analysis and management platform for high-content screening and cell imaging data. Quickly process, analyze, share, and store the vast volumes of data generated by high-content screening and cellular imaging, including live cell imaging, 3D imaging, ...

Signals VitroVivo™

Signals VitroVivo is an intuitive, configurable, flexible screening workflow processor coupled with the unparalleled data visualization and analysis capabilities of TIBCO Spotfire.