Functional genomic screens enable the modulation of hundreds or thousands of genes in a single experiment to identify genetic pathways, cellular processes, novel therapeutic targets, and to genetically profile existing or potential therapeutics.
PerkinElmer’s wide range of CRISPR solutions are designed to support you in every step of your CRISPR workflow. From CRISPR and RNAi libraries for high-throughput functional genomic screening to automated image platforms and cellular assays, we offer all the tools and technologies to help you edit with confidence and maximize your chance of success.
Gene expression can be regulated using either RNAi or CRISPR technology, where RNAi represses gene expression at the mRNA level (knockdown), while CRISPR works at the DNA level and can permanently knockout, modulate, or knock-in genes.
There are two main screening approaches, pooled and arrayed. Explore here how they work.
|Pooled Screens||Arrayed Screens|
|Pooled screening: whereby editing is performed with a mixture of guide RNA (gRNA) and thus a high number of perturbations occur. The mixed cell population is then exposed to a treatment and analysed by NGS for deconvolution with an optional sorting step before. This approach is very cost effective, however only macro effects can be detected.||Arrayed screens: where each CRISPR-based perturbation occurs in a separate well of a multi-well plate, offering a clear phenotype to genotype correlation. The main read-out is imaging, which could be combined with detection or sequencing enabling a multi-parametric analysis. They are also well-suited for more advanced cell models like primary cells or 3D cell cultures.|
CRISPR has exploded within the scientific research community and contributed to many notable breakthroughs. With a ride range of important applications, the technology has become pivotal for research and beyond.
Enables loss of function studies: screening with complete loss of gene expression provides the maximal window for phenotypic effect and high statistical power for hit discovery.
This homology-directed repair (HDR) introducing or correcting a SNP mutation, or adding a reporter tag to an endogenous gene.
Represses expression rather than completely knocking out the target gene and is ideally suited to study drugability and to evaluate the function of genes that when knocked-out are essential or that are amplified.
Amplifies gene expression in its endogenous context and enables for the first time, to study activation-linked responses on a genome-wide level.
Selecting the appropriate format of CRISPR reagents is the first critical step before starting a screen. We offer a variety of formats, so that regardless of the scientific question, we can support your experimental needs.
Cas9 nuclease reagents are available as Cas9 protein, mRNA, plasmid, or lentiviral Cas9. For optimization and enrichment, versions co-expressing a fluorescent tag are available.
Edit-R ™ CRISPR guide RNA are available in pools or as individual reagents, as predefined CRISPR knockout libraries, or a library can be designed using the cherry-pick library tool.
HDR relies on the presence of a donor template with sufficient homology to the regions flanking the cut site. The homology arms’ length and donor type will depend on the modification being made. Our templates make it easy to customize a donor oligo.
Successful introduction of gRNA and Cas9 protein into the cell is key to any gene-editing experiment to ensure efficiency. Our range of transfection reagents provide efficient and reliable delivery of CRISPR gRNA with minimal cellular toxicity.
These types of assays are often labor intensive and require many steps for re-suspending the CRISPR reagents, cell transfer, and treatment addition or staining. To accelerate your assay, we offer a full range of liquid handling, integrated laboratory automation, and cell counters that together with our specially designed microplates, improve your workflow and provide consistent and quality results.
For research use only. Not for use in diagnostic procedures.
The JANUS® G3 automated liquid handling workstations offer flexible automated sample preparation solutions to meet your specific application needs. The JANUS G3 portfolio includes both dedicated, application-specific workstations and personalized automated solutions. The JANUS G3 workstation can be ...
从细胞筛查和细胞成像应用到高通量筛查、基因组学以及基于DNA/RNA的筛查，我们提供各种以应用为重点的集成式机械臂解决方案来优化微孔板处理、液体处理和检测。 我们的多功能微孔板处理能力能够实现简单的集成，即使最常规的工作流程也能立即加速。结合我们的plate::works™自动化控制和日程安排软件，我们能够让用户通过直观的界面引导以及日程安排软件完全控制他们的自动化流程。 利用我们一流的高通量试剂、多模式微孔板检测、高含量分析和液体处理产品组合，PerkinElmer拥有独特的定位：了解您的基础科学和应用需求。您可以依靠我们来提供解决方案以解决您全面的科学要求。这一切都来自同一个供应商，为您提 ...
Choosing the right microplate is a critical, often overlooked, part of an assay. The right microplate helps provides valuable data, whereas the wrong microplate can lead to missed or inaccurate data leading to missed project timelines and ultimately higher costs. New technologies for drug developmen ...
Nexcelom offers a broad range of slide or plate-based automated cell counters featuring brightfield and fluorescent read-out capabilities.
Once it's time to acquire an image, options for read-outs can range from simple phenotypic or viability assays to multiparametric high-content analysis. Our line of sensitive yet powerful microplate readers, live cell imagers, and high-content analysis systems, plus innovative reagents enable you to accelerate your workflow and reduce costs.
用常规方法进行蛋白和核酸的准确定量分析是比较困难的。我们的TSA试剂盒可以大幅提升免疫组化、免疫荧光和原位杂交的信号强度，并且对检测设备无特殊要求。 我们也可以在单张图像上实现七色（含DAPI）复染的多标记蛋白检测。其工作流程与标准的免疫组化方案类似，而且不必担心抗体物种来源造成的交叉干扰，全部选用效率最高的抗体完成多色标记。常见的标记例如CD20, CD4, CD45RO, CD68, FOXP3, panCK等等。 仅限于科研使用，不用于临床诊断。
High-Content Analysis (HCA) or High-Content Screening (HCS) combines high-throughput automated imaging and analysis to extract quantitative multi-parametric data at the single-cell level. Originally developed as a complementary technology to traditional biochemical high-throughput screening (HTS) in ...
我们提供适合任何实验室各种应用需求的多模式微孔板读板仪。每一款读板仪都具有业内领先的检测技术，因此无论您选择任何一款仪器，您都可获得针对您应用的最佳检测性能。 VICTOR X 微孔板读板仪 市场上第一台多功能微孔板读板仪，VICTOR™ X 系列具有多种检测功能，性能可靠，为各种规模的实验室提供集灵活性、高速度和高性能于一体的解决方案。 EnSpire 微孔板读板仪 第一台整合了Corning® Epic® 无标记技术的桌面式多功能读板仪，EnSpire® 专为多用户科研环境设计，提供高性能检测技术，且在保证灵敏度的前提下进行各种灵活的实验应用。 EnSight 微孔板读板仪 第一台整合了多 ...
Drug discovery is a complex, costly, and time-consuming undertaking. And for research scientists, high-throughput screening is where the journey to discovery begins. Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our HTS scr ...
The Celigo image cytometry system provides high-throughput, whole-well imaging and quantitative data through image analysis in bright field and up to four fluorescent channels, for a wide variety of cell-based assays.
Nexcelom Bioscience offers a wide range of fluorescent reagents and kits for cell counting and cell-based assays. Our reagents are optimized to work with Cellometer and Celigo imaging systems as well as other fluorescence-based instruments.
Once your gene-editing experiment is complete, you’re faced with readouts and large amounts of data that need to be analyzed. Our next generation informatics solutions help you to contextually analyze all that data, so you have more physiologically relevant results to make more informed decisions.
Signals Image Artist™ is our next generation image analysis and management platform for high-content screening and cell imaging data. Quickly process, analyze, share, and store the vast volumes of data generated by high-content screening and cellular imaging, including live cell imaging, 3D imaging, ...
Signals VitroVivo is an intuitive, configurable, flexible screening workflow processor coupled with the unparalleled data visualization and analysis capabilities of TIBCO Spotfire.