Functional Genomic Screening

YOUR PATHWAY TOWARD CRISPR WORKFLOWS

Functional genomic screens enable the modulation of hundreds or thousands of genes in a single experiment to identify genetic pathways, cellular processes, novel therapeutic targets, and to genetically profile existing or potential therapeutics.

PerkinElmer’s wide range of CRISPR solutions are designed to support you in every step of your CRISPR workflow. From CRISPR and RNAi libraries for high-throughput functional genomic screening to automated image platforms and cellular assays, we offer all the tools and technologies to help you edit with confidence and maximize your chance of success.

Functional Genomic Workflows

Gene expression can be regulated using either RNAi or CRISPR technology, where RNAi represses gene expression at the mRNA level (knockdown), while CRISPR works at the DNA level and can permanently knockout, modulate, or knock-in genes.

There are two main screening approaches, pooled and arrayed. Explore here how they work.

A Better Understanding Starts Here

Explore by Application 

CRISPR has exploded within the scientific research community and contributed to many notable breakthroughs. With a ride range of important applications, the technology has become pivotal for research and beyond. 

Disease Models

• Use CRISPR knockout, CRISPR activation, and CRISPR interference to understand specific gene contribution to a disease
• Use gene knock-in to insert mutations mimicking disease or wild-type biology for better validated targets or compound identification

Pathway Analysis

• Understand the function of genes and how they contribute to biological processes and diseases

Target Identification

• Identify relevant targets or hits from phenotypic screening
• Assess the contribution of each gene in the genome to compound activity in a full genome loss-of-function screen

Target Validation

• Verify targets in an arrayed secondary screen (smaller set of genes) and eliminate false positives
• Investigate gene knockout or modification in a variety of cell types to determine if a gene target provides the same phenotype in multiple cell types

Cell & Gene Therapy

• Reverse disease-causing mutations
• Edit CAR-T cells to attack cancer cells

The Various Types of CRISPR

CRISPR Knockout

Enables loss of function studies: screening with complete loss of gene expression provides the maximal window for phenotypic effect and high statistical power for hit discovery.

CRISPR Knock-in

This homology-directed repair (HDR) introducing or correcting a SNP mutation, or adding a reporter tag to an endogenous gene.

CRISPRi Screening

Represses expression rather than completely knocking out the target gene and is ideally suited to study drugability and to evaluate the function of genes that when knocked-out are essential or that are amplified.

CRISPRa Screening

Amplifies gene expression in its endogenous context and enables for the first time, to study activation-linked responses on a genome-wide level.