DELFIA® (Dissociation-Enhanced Lanthanide Fluorescent Immunoassay) TRF technology combines the benefits of Time Resolved Fluorescence (TRF) with the use of lanthanide chelates for a highly sensitive, robust and reproducible assay format. With over 20 years of history, and thousands of publications, DEFLIA is ideal for simple to complex matrices and can be used for both biochemical and cell-based assays.
The use of long-lifetime fluorescent lanthanide chelates, rather than other fluorophores, allows for a delay in measurement between excitation and emissions, which occur simultaneously in standard fluorometric assays. By combining the benefits of TRF with the use of lanthanide chelates, DELFIA offers multiple advantages over standard fluorometric detection:
- High sensitivity (femtogram level detection)
- Wide dynamic range (4-5 log)
- High S:B
- Large Stokes shift
- Superior stability – (days)
- Multiplexing capability
From immunoassays, to binding and enzymatic studies, to cytotoxicity and cell proliferation assays, our DELFIA TRF assays are sure to provide you with unmatched sensitivity, time and time again.