PerkinElmer

ELISA Amplification

Enzyme-linked immunosorbent assay (ELISA) is a sensitive immunoassay technique that uses an enzyme linked to an antibody or antigen as a marker for the detection of a specific protein, such as an antigen or antibody. ELISAs are utilized to detect or quantitate analytes in a sample.

PerkinElmer has developed an HIV-1 p24 Antigen ELISA Kit for the detection of HIV-1 and HIV-0 subtypes in various sample matrices and ELISA amplification kits (ELAST®) to improve sensitivity and reduce antibody consumption.

PerkinElmer’s ELAST (ELISA Amplification System) is designed to amplify the signal generated by the enzyme horseradish peroxidase (HRP) in solid phase analytical methods such as ELISA. ELAST is based on the use of PerkinElmer’s proprietary Tyramide Signal Amplification (TSA) technology.

Combined with our HIV-1 p24 Antigen ELISA Kit, the ELAST p24 ELISA Amplification System enhances HIV-1 p24 Antigen ELISA sensitivity. The assay is based on the conventional ELISA technique with an added signal amplification step (ELAST). The ELAST ELISA Amplification system is also available to reduce antibody consumption of detection antibodies in HRP- based ELISAs.

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