High Content Screening Instruments

High-Content Analysis (HCA) or High-Content Screening (HCS) combines high-throughput automated imaging and analysis to extract quantitative multi-parametric data at the single-cell level. Originally developed as a complementary technology to traditional biochemical high-throughput screening (HTS) in drug discovery, today high-content screening is established in a far broader area of the life science space as an unbiased imaging method to assess cellular function.

High-Content Imaging (HCI) is typically defined as lower throughput automated microscopy (<100,000 samples or data points) compared to high-content screening. However, high-content imaging is often used interchangeably with the terms high-content screening and high-content analysis.1

High-content screening offers a number of advantages over traditional techniques:

  • More information from cellular samples
    Fully automated imaging and unbiased quantitative image analysis exploits the full potential of microscopy, improving your lab’s throughput and productivity.
  • More meaningful results
    Screening can be performed in more physiologically and biologically relevant systems than in classical biochemical assays.
  • More questions answered simultaneously
    Data sets are more information-rich compared to non-imaging read-outs, allowing a number of questions to be answered simultaneously.


  • Buchser, William, et al. "Assay development guidelines for image-based high content screening, high content analysis and high content imaging." Assay guidance manual [Internet] (2014).



High-Content Screening System Technologies



Confocal Imaging

Spinning disk confocal microscopy offers several advantages over conventional optical microscopy, including widefield and laser scanning confocal microscopy.


Water Immersion Objectives

Proprietary automated water-immersion objectives with very high numerical aperture deliver and capture more photons and provide a higher resolution in XYZ than conventional objectives.



Stability, brightness and spectral fit for excitation are key considerations for illumination sources in fluorescent imaging.


sCMOS Cameras

The large-format sCMOS camera – one in Operetta CLS or up to four of them in the Opera Phenix - delivers low noise, wide dynamic range, and high resolution – perfect for sensitive and quantitative measurements at short exposure times.

Intelligent Image Acquisition

More accurately target your object of interest for significantly reduced acquisition and analysis times with intelligent acquisition technology for HCS.


Machine Learning

Machine learning techniques enable supervised and unsupervised feature selection and clustering of highly multi-parametric data sets.


Operetta CLS High Content Analysis System

Operetta® CLS is a high throughput microplate imager for high-content analysis (HCA). It can acquire, analyze and manage fluorescence, brightfield and digital phase contrast images.


Opera Phenix Plus High-Content Screening System

The Opera Phenix® Plus High-Content Screening System is the premier confocal solution for today's most demanding high content applications.


High-Content Screening System Filters and Lenses

Enable sensitive imaging on our cellular vivo imaging systems -- at whatever resolution you desire. With our custom-designed optics, including optical filters, objectives and zoom lenses, you have the ideal view for any application.


Meet Our Users

Read how scientists around the globe are using our high-content screening products and services to advance their research in a variety of fields.


Key Content

Operetta CLS High-Content Analysis System Brochure

The product of more than a decade of experience in high-content screening, the Operetta CLS delivers the speed and sensitivity for everyday assays, and even com ...


Opera Phenix Plus 高内涵筛选系统手册

The Opera Phenix® Plus High-Content Screening System is the premier confocal solution for today's most demanding high content applications.





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