PerkinElmer

Immunoassays

Since their introduction in the 1950’s, immunoassays have been an integral part of research and drug discovery. Over the years, immunoassay technologies and formats have expanded, leading to the plethora of immunoassay technology platform options we now have today.

Discover our range of immunoassay technologies that enable the selection of optimal immunoassay platforms for your requirements. Choose from traditional wash-based assays to homogenous kits and toolboxes.  From sample compatibility to sample volume, throughput, detectable range, signal window, signal stability, reproducibility, automation compatibility, and desired speed of results, we offer a vast selection of immunoassay technology platforms, pre-configured kits, and customizable reagents for superior results.

Benefits of our Immunoassay Platform Solutions:

  • Wide dynamic range
  • High sensitivity
  • Reduced background noise
  • Large stokes shift
  • Extended signal stability
  • Faster results
  • Multiple detection systems


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  Alpha DELFIA TRF ELISA HTRF and LANCE Ultra TR-FRET LEGENDplex
Plate Format 96- to 1536-wells 96- to 384-wells 96-wells 96- to 1536-wells Flexible
Miniaturization Yes Limited Limited Yes Yes
Time to Results 1-2 Hours 4-6 Hours 1.5-20 Hours 1-2 Hours 4 Hours
Wash Steps No Yes Yes No Yes
Dynamic Range 5 Log 5 Log 2 Log 4 Log 4 Log
Avidity Yes No No Only with Streptravidin No
Signal Stability Up to 24 Hours Days - Years Up to 1 Hour 24 Hours - Days Up to 24 Hours
Multiplexing Yes (Up to 3 Targets) Yes (Up to 3 Targets) No No Yes (Up to 14 Targets)
Reader Requirements Alpha Compatible TRF Compatible Colorimetric Reaction TRF Compatible Flow Cytometer with Red and Blue/Yellow-Green Lasers
Chemistry Fluorophore Embedded Beads Fluorophore Chelate

N/A

 Fluorophore Cyrptate (HTRF)

Fluorophore Chelate (LANCE Ultra)		 N/A

 

 

Need help choosing the immunoassay platform solution fit for your research? Check out our Selection Guide!

 

Alpha

Alpha Technology

 

Alpha is a bead-based, no-wash luminescent technology. When Alpha Donor and Acceptor beads are brought together through binding to target, a cascade of chemical reactions is set in motion, creating an amplified signal. These versatile beads are conjugated with various biomolecules that enable detection of unique biological events, offering maximum flexibility. Alpha technology is compatible with a wide range of sample types ranging from simple buffers, cell supernatants, and cell lysates to complex tissue, serum, plasma, and other biological samples.

Alpha offers benefits including:

  • Faster time to results
  • Ability to quantitate large protein complexes, nucleosomes, and viral particles
  • Option for multiplexing

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DELFIA® TRF

DELFIA TRF

DELFIA® TRF is a proven, robust wash-based immunodetection platform with over 20 years of history. DELFIA has a similar assay principle and workflow to that of a traditional ELISA, but with the added benefits of a stable, time-resolved fluorescent signal improved assay dynamic range. DELFIA also offers the ability for multiplexing. DELFIA TRF is compatible with the widest range of sample types, ranging from simple buffers, cell supernatants, and cell lysates to complex biological samples, including tissue, serum, plasma, and blood.

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ELISA

Focus on a single target with BioLegend’s ELISA kits and sets. Find all the reagents for your ELISA workflow with our economical kits, antibodies, and ancillary reagents. BioLegend offers four types of ELISA kits, including easy-to-use LEGEND MAX™ kits with pre-coated plates and RAPID MAX™ kits that cut your assay time down to 90 minutes (watch the protocol video to learn how time is saved). In addition, BioLegend provides reliable antibody pairs, ancillary reagents, and standards for those who want to develop their own ELISA assays.

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HTRF®

HTRF

 

HTRF® (Homogeneous Time Resolved Technology) is a FRET-based, no-wash technology.

Rapid, homogeneous, easy to use and to automate, allowing extreme miniaturization and easy assay development – over time, HTRF has become the gold reference for time-resolved FRET.

HTRF technology can be used in competitive and noncompetitive formats, performed as cellular or biochemical assays and thus can been applied to a variety of applications including GPCRs, kinases, epigenetics, and quantification of a range of biomarkers including cytokines.

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LANCE® Ultra™ TR-FRET

LANCE Ultra TR-FRET

 

LANCE® Ultra TR-FRET is a no-wash proximity assay technology that combines the benefits of time resolution (TR) with fluorescence resonance energy transfer (FRET) principles by using energy donor and energy acceptor fluorophore labels. It’s everything you love about TRF detection with fastest results and no wash steps. LANCE Ultra TR-FRET is compatible with buffer, cellular supernatant and cell lysate samples.

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LEGENDplex™

LEGENDplex™ bead-based immunoassays work on the same principle as a sandwich ELISA, with the advantage that they can simultaneously quantify up to 14 targets in a single sample using only a flow cytometer—no other instruments are needed. Data can be analyzed with our free LEGENDplex™ Data Analysis Software Suite. Choose from dozens of existing panels or customize your own.

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Immunoassays Spotlight

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Detecting Cytokines with AlphaLISA Guide
Cytokines are implicated in the pathology and outcome of disease states across all therapeutic areas. When preparing to run cytokine assays, it is key to consid ...
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AlphaLISA® SureFire® Ultra™ Guide
AlphaLISA SureFire Ultra assays provide a reliable and robust method for quantifying a targeted phosphorylation event in cell-based experiments.
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HTRF-Immunoassay-Technology-Platform.jpg

HTRF Technology Basics
Rapid, homogeneous, easy to use and to automate, allowing extreme miniaturization and easy assay development – discover how HTRF can benefit your research.
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Immunoassay-Technology-Platforms-Video.jpg

Alpha, DELFIA, HTRF, & LANCE Platform Video
This video presents how our platforms work and help scientists in their research to explore biological interactions.
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RAPID MAX™ ELISA Comparison Video
BioLegend’s RAPID MAX™ kits cut assay time to 90 mins and offer the same performance parameters as typical ELISA kits.
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LEGENDplex-Multiplex-Platform-Video.jpg

LEGENDplex Protocol Video
Want to watch a video that details the laboratory portion of the LEGENDplex™ protocol? Check out this peer-reviewed article published with the Journal of Visual ...
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Explore Alpha, TRF, and TR-FRET by Therapeutic Area

REAGENT_500x500

Virology Reagents
Viruses and virally transmitted diseases have been impacting human health and well-being for millennia. Some of our greatest scientific challenges and medical t ...

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Neuroscience Research Reagents

Neuroscience Research Reagents
Over 55 million people worldwide are developing dementia and this number is expected to double by 2050. All the neurodegenerative disorders studies and associat ...

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Immuno-Oncology Reagents

Immuno-oncology Reagents
Immuno-oncology is a leader in the worldwide drug development pipeline and is at the forefront of cancer research. By giving the immune system tools to detect t ...

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inflammation_320x320

炎症
Detect disease-causing microbes and quantitate biomarkers of inflammation with our wide range of in vivo, cellular, in situ, and biochemical research products. ...

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Explore Alpha, TRF, and TR-FRET by Target Area

Target Engagement Reagents
The pharmacological validation of the interaction of a ligand with its protein target is referred to as target engagement. Target engagement studies are crucial ...

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Cytokine Research Reagents

Cytokine Research Reagents
Cytokines regulate several physiological and pathological roles including innate immunity, acquired immunity, and many inflammatory responses. PerkinElmer offer ...

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Targeted Protein Degradation Assays and Reagents

Targeted Protein Degradation Assays & Reagents
Protein homeostasis in the cellular environment is achieved through the ubiquitin-proteasome system (UPS) and the autophagy-lysosomal system (ALS). Together, th ...

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Biomarker Detection Reagents

生物标志物检测
从试验立项到初级和二级筛选，生物标记物分析过程中，珀金埃尔默的生物标记检测试剂和试剂盒具有高灵敏度、较宽的动态范围且便于实现自动化。 与市场上所有的ELISA或TR-FRET分析相比，由于无需洗涤及分离步骤，我们的均质技术能够最快的获得试验结果。

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Protein Kinase Research Reagents

激酶
Cancer, diabetes, and Alzheimer’s research can benefit from the study of phosphorylation and protein kinase activity. Because of their role in signal transducti ...

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Epigenetic Research Reagents

表观遗传学
To help unravel the complex biology of epigenetics, we provide a suite of enabling reagents and technologies that shorten assay development time and accelerate ...

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GPCR Research Reagents

GPCR
G蛋白偶联受体（GPCR）是结构变化多样的一大类蛋白膜受体，包括：β1和β2肾上腺素能受体、多巴胺能受体和胆碱能受体。我们可提供G蛋白偶联受体研究所需的所有先进检测分析设备。 以及多种靶标Gs-、Gq-和Gi-偶联受体的实验试剂。 我们产品还包括： 荧光、发光或吸收光检测所需的可调焦GPCR细胞成像仪和液体工作站； 经 ...

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PK_Research

Protein Kinase Research
Protein kinases are key focus targets in drug discovery. Kinases regulate most cellular pathways and are responsible for the modification of nearly a third of a ...

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Protein-Protein Interaction Research Reagents

蛋白质 - 蛋白质相互作用
Accurately measure the function of proteins and gain new insights into protein-protein interaction (PPI) to modulate cellular processes. Inhibition and stabiliz ...

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Featured Products

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微孔板
Choosing the right microplate is a critical, often overlooked, part of an assay. The right microplate helps provides valuable data, whereas the wrong microplate ...

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仪器

EnVision Nexus Multimode Microplate Reader

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HTRF Detection kit

HTRF Human Total ATG14 Detection Kit
This HTRF kit enables the cell-based quantitative detection of ATG14 as a readout of the autophagy pathway, and can be combined with our Phospho-ATG14 Ser 29 ki ...

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Mini ELISA Plate Reader

Mini ELISA Plate Reader
Reading your ELISA should be as streamlined as the rest of your ELISA workflow.

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