PerkinElmer

LANCE TR-FRET

OF

LANCE® (Lanthanide Chelate Excite) TR-FRET technology combines the benefits of Time Resolved Fluorescence (TRF) with those of Förster Resonance Energy Transfer (FRET) in a simple, highly-sensitive, and highly reproducible assay platform for detection, quantitation and screening in microplate format.

The use of long-lifetime fluorescent lanthanide chelates, rather than other fluorophores, allows for a delay in measurement between excitation and emissions, which occur simultaneously in standard fluorometric assays. Furthermore, FRET detection utilizes energy donor and energy acceptor fluorophore labels in solution to create a no-wash detection assay. By combining the benefits of TRF and FRET, LANCE TR-FRET offers a multitude of benefits over standard fluorometric detection:

  • Homogeneous assay format (no wash steps, no separation steps)
  • Reduced background leading to better S:B
  • Large Stoke’s shift
  • Wider dynamic range
  • Extended signal stability
  • Increased sensitivity. Lower detection limits of 10 pg/mL compared to 100 pg/mL for standard fluorometric detection
  • Faster time to results

From biomarker detection assays, to cAMP/GPCR studies, to kinase and epigenetic assays, our LANCE® and LANCE Ultra TR-FRET assays are sure to save you time and hassle.

OnPoint Services
OnPoint Services

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can label your assay components with LANCE donor and acceptor molecules. Or maybe you are you short on time and need an assay developed quickly? Custom LANCE assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.

推荐产品

How can we help you?

When it comes to developing assays or troubleshooting issues, always know you’re never on your own. With over 30 years’ experience in defining, synthesizing and optimizing chelate and chelate-based bioanalytical assays PerkinElmer is here to help you succeed. Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.

FAS: Our global team of Field Application Scientists are hands-on subject matter experts in assay development. They are available to assist in both assay development and troubleshooting.

Application Support Knowledgebase (ASK): Need help at 1am? We’ve got it covered. The Application Support Knowledgebase (ASK) contains detailed information on assay development, protocols, tips, FAQs, citations, troubleshooting and more. Accessible 24 hours a day, 7 days a week on our website. http://www.perkinelmer.com/ASK/LANCE.

  • 产品与服务

  • 资源,活动及更多信息

缩小结果
抗体偶联物
  • Streptavidin (5)
  • Anti-mouse IgG (3)
  • Anti-FLAG (3)
  • Anti-GST (3)
  • Anti-rabbit IgG (3)
  • Anti-6X His (3)
  • Anti-C-tag (2)
  • Anti-c-Myc (2)
  • Anti-FITC (2)
  • Anti-human IgG (2)
  • Anti-V5 (2)
  • Anti-phospho-Histone H3 (2)
  • Anti-methyl arginine (1)
  • Anti-H3K9me2 (1)
  • Anti-phospho-PKC (1)
  • Anti-HA (1)
  • Anti-phospho-rpS6 (1)
  • Anti-phospho-Crosstide (1)
  • Anti-phospho-Tyrosine Hydroxylase (1)
  • Anti-H3K36me2 (1)
  • Anti-H3K27me2 (1)
  • Anti-O-linked-GlcNAc (1)
  • Anti-phospho-Acetyl-CoA-Carboxylase (1)
  • Anti-phospho-PLK (1)
  • Anti-H3K4 (1)
  • Anti-H4R3me (1)
  • Anti-phospho-4EBP1 (1)
  • Anti-phospho-p70S6K (1)
  • Protein A (1)
  • Anti-p53K382ac (1)
  • Anti-P-Ser 14-3-3 (1)
  • Anti-H3K27ac (1)
  • Anti-phospho-glycogen synthase (1)
  • Anti-phospho-threonine (1)
  • Anti-P-Tyr-100 (1)
  • Anti-H3K9ac (1)
  • Anti-phospho-Topo IIa (1)
  • Anti-phospho-I?Ba (1)
  • Biotin (1)
  • Protein G (1)
  • PT66 (1)
  • PY20 (1)
  • Anti-H3K4me1-2 (1)
  • Anti-H3K27me3 (1)
  • Anti-H3K9/H3K27 (1)
  • Anti-acetyl-lysine (1)
  • Anti-phospho-MEK1/2 (1)
应用
  • Protein Analysis & Detection (238)
  • Kinase (82)
  • Protein-protein interactions (22)
  • Epigenetics (14)
  • Enzymatic (6)
  • cAMP (2)
检测点(200-50,000)
  • 10000 (128)
  • 500 (125)
  • 5000 (4)
检测目标
  • IgG (8)
  • EGFR (5)
  • IL6 (5)
  • Leptin (4)
  • VEGF-A (4)
  • IFNγ (4)
  • IGF1 (4)
  • FCGR3A (4)
  • TNFα (4)
  • Akt1 (3)
  • CCL2 (3)
  • EGF (3)
  • IL17A (3)
  • IL1β (3)
  • STAT1 (3)
  • CRP (2)
  • Lambda light chain (2)
  • Src (2)
  • MANF (2)
  • IgA (2)
  • PER.C6 HCP (2)
  • C-peptide (2)
  • TBK1 (2)
  • PTEN (2)
  • hCG (2)
  • Albumin (2)
  • Adiponectin (2)
  • ANGPTL3 (2)
  • IL8 (2)
  • p53 (2)
  • PD-1 (2)
  • IL2 (2)
  • IL1α (2)
  • CXCL1 (2)
  • ApoC3 (2)
  • Insulin (2)
  • ApoA1 (2)
  • Glucagon (2)
  • IL-23A (2)
  • ANGPTL4 (2)
  • Protein A (2)
  • ERK1/2 (2)
  • BTK (2)
  • CCL4 (2)
  • Cortisol (2)
  • COX-2 (2)
  • PIP Collagen (2)
  • Fibrinogen (2)
  • IL-31 (2)
  • Oncostatin M (2)
  • MMP12 (2)
  • c-Myc (2)
  • IL17F (2)
  • CCL3 (2)
  • Fibronectin (2)
  • Bcl2 (2)
  • Residual DNA (2)
  • MMP8 (2)
  • MMP3 (2)
  • Zap70 (2)
  • IFNα (2)
  • Frataxin (2)
  • COL1A1 (2)
  • IL-1RA (2)
  • Kappa light chain (2)
  • ApoE (2)
  • TDP-43 (2)
  • IgM (2)
  • FSH (2)
  • GLP-1 (2)
  • GAD67 (2)
  • IL12 (2)
  • IgE (2)
  • MIP1α (2)
  • IL28B (2)
  • ErbB2 (2)
  • cAMP (2)
  • SMAD3 (2)
  • Perlecan (2)
  • CXCL9 (2)
  • E.coli HCP (2)
  • ANGPT1 (2)
  • Nidogen-1 (2)
  • LC3B (2)
  • MMP7 (2)
  • IFN-λ2 (2)
  • TSH (2)
  • STAT3 (2)
  • NFκB (2)
  • PD-L1 (2)
  • Aβ1-40 (2)
  • CCL5 (2)
  • SLP-76 (2)
  • Tau (2)
  • TREM2 (2)
  • Akt1/2/3 (2)
  • PAI-1 (2)
  • CXCL10 (2)
  • IL4 (2)
  • E-Cadherin (2)
  • CHO-P (1)
  • AMPKα (1)
  • STAT5 (1)
  • SMAD2 (1)
  • IL5 (1)
  • PCSK9 (1)
  • tPA (1)
  • eIF2α (1)
  • IL11 (1)
  • LCK (1)
  • MBNL1 (1)
  • CHO HCP (1)
检测目标类
  • Protein (102)
  • Cytokine (53)
  • Phosphoprotein (20)
  • Antibody (18)
  • Hormone (16)
  • Peptide (10)
  • Fc-receptor (4)
  • Receptor (2)
  • DNA (2)
自动化兼容
  • Yes (353)
检测方法
  • Time-Resolved Fluorescence (TRF) (319)
  • TR-FRET (319)
实验类型
  • In vitro (277)
荧光基团
  • Eu-W1024 (51)
  • ULight (30)
  • SureLight APC (6)
  • Eu-W1024-ITC (2)
  • Eu-W1284-Iodoacetamido (2)
  • SureLight-APC (1)
  • Eu-W8044-DTA (1)
  • Eu-W8044 (1)
格式
  • Microplates (281)
分子修饰
  • Phosphorylation (41)
  • Methylation (9)
  • Acetylation (6)
  • GlcNAc (1)
产品品牌名称
  • LANCE Ultra (253)
  • LANCE Ultra (51)
  • LANCE (27)
  • LANCE SureLight (7)
目标种
  • Human (191)
  • Mouse (16)
  • Bovine (2)
  • Rat (2)
  • CHO (2)
  • Rabbit (2)
治疗领域
  • Inflammation (53)
  • Cancer (39)
  • Metabolic (36)
  • Biologics/Bioprocess (30)
  • Angiogenesis (11)
  • Central Nervous System (9)
  • Autophagy (2)
  • CNS & Neurologic (1)
  • Cardiovascular (1)

"LANCE TR-FRET" 1-50363 产品与服务

每页
PerkinElmer

Leptin (mouse), lyophilized (LANCE)

Mouse leptin standard, lyophilized, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Mouse Leptin Detection Kit, but can be ordered separately.

ULight anti-FITC

ULight™ labeled anti-FITC antibody for LANCE® TR-FRET assays. The anti-FITC antibody is a purified mouse IgG2a monoclonal antibody.
PerkinElmer

IFN-γ (human), lyophilized (LANCE)

Human IFNγ standard, lyophilized, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human IFNγ Detection Kit, but can be ordered separately.

IL-6 (mouse) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse IL-6 Detection Kit is designed for detection and quantitation of murine IL-6 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

ULight anti-rabbit IgG

ULight™ labeled anti-rabbit IgG antibody for LANCE® TR-FRET assays. The anti-rabbit IgG antibody is a purified mouse polyclonal antibody.

Adiponectin (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Adiponectin Detection Kit is designed for detection and quantitation of human adiponectin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
PerkinElmer

LANCE Ultra STAT3 Positive Control Lysate

Control lysates for LANCE Ultra TR-FRET cellular phospho-STAT3 and total STAT3 detection assays.
Protein

IGF-1 (rat), lyophilized (LANCE)

Rat IGF-1 (insulin-like growth factor 1) standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra rat IGF-1 Detection Kit, but can also be ordered separately.
LANCE Detection Kit

IL-1α (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IL-1α Detection Kit is designed for detection and quantitation of human interleuukin-1α in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
AlphaLisa Analyte 2

PIP Collagen (human), lyophilized (LANCE)

PIP collagen standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra PIP Collagen TR-FRET Detection Kit, but can also be ordered separately.

ULight anti-c-myc antibody

ULight labeled anti-c-myc antibody for LANCE® TR-FRET assays.
PerkinElmer

Ultra HiBlock Buffer 5X

This product is designed to be used in combination with LANCE Ultra TR-FRET biomarker detection kits. The Ultra HiBlock buffer was specially developed buffer to block non-specific protein binding sites.

LANCE Ultra ULight-Glycogen Synthase (Ser641/pSer657) Peptide

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. Glycogen synthase peptide (Sequence: PASVPPSPSLSRHSSPHQ(pS)ED) labeled with ULight dye. Synthetic peptide containing residues surrounding Ser641 and Ser657 of human Muscle Glycogen Synthase. This peptide is pre-phosphorylated on Ser657. Target phosphorylation site: Ser641.
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total STAT3 LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra total STAT3 kits are designed for the detection of total STAT3 (phosphorylated and unphosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-STAT3, and is compatible with both adherent and suspension cells.

TNFα (mouse) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse TNFα Detection Kit is designed for detection and quantitation of mouse tumor necrosis factor α in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Protein

PD-1 (human), lyophilized (LANCE)

Human PD-1 standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human PD-1 TR-FRET Detection Kit, but can also be ordered separately.
LANCE Detection Kit

IGF-1 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IGF-1 Detection Kit is designed for detection and quantitation of Human IGF-1 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
PerkinElmer

LANCE Ultra hIL-11,lyophilized(0.3ug)

LANCE Ultra hIL-11,lyophilized(0.3ug)

IL-17A (mouse/rat) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse/Rat IL-17A Detection Kit is designed for detection and quantitation of mouse or rat interleukin 17A in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Protein

Apo-CIII (human), lyophilized (LANCE)

Human Apo-CIII standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra ApoC3 Detection Kit, but can also be ordered separately.

E cadherin (human) LANCE Ultra TR-FRET Detection Kit

Human E-cadherin standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human E-cadherin TR-FRET Detection Kit, but can also be ordered separately.

ULight Anti-C-tag

ULight™ labeled anti-C-tag antibodies for LANCE® TR-FRET assays. This mouse monoclonal IgG1 antibody recognizes N-terminal and C-terminal Protein C tagged fusion proteins. The anti-C-tag antibody can be used to capture C-tag proteins, and can be used in conjunction with LANCE Europium-labeled reagents to create LANCE TR-FRET assays.

Leptin (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Leptin Detection Kit is designed for detection and quantitation of human leptin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

TREM2 LANCE Ultra TR-FRET Detection Kit

The LANCE Ultra TREM2 detection kit is designed for the detection of TREM2 in cell supernatants using a simple, homogeneous LANCE Ultra assay (no wash steps).

LANCE Ultra ULight-p70 S6K (Thr389) Peptide

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Thr389 of human p70 S6K. The ULight-p70 S6K peptide can be phosphorylated at Thr389 by several kinases including mTOR, COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1, and TAOK2. Phosphorylation motif: LGFTYVAP
LANCE Detection Kit

GAD67 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human GAD67 Detection Kit is designed for detection and quantitation of Glutamic acid decarboxylase 67 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
PerkinElmer

IL-6 (human), lyophilized (LANCE)

Human Interleukin 6 (IL-6) standard, lyophilized, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra human IL-6 Detection Kit, but can be ordered separately.
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-AKT 1/2/3 (Ser473) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-AKT1/2/3 (Ser473) kits are designed for the detection of AKT1, AKT2, and AKT3 (phosphorylated at residue S473) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

LANCE Ultra Europium-anti-methyl-Histone H3 Lysine 4 (H3K4me1-2)

Europium-labeled monoclonal antibody recognizing human H3 mono- and di-methylated at lysine 4 (H3K4me1-2). For use in LANCE® Ultra epigenetic writer and eraser assays.

LANCE Ultra Europium-anti-phospho-CREB (Ser133)

Monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Ser133 in the cAMP response element binding (CREB) protein. CREB (Ser 133) can be phosphorylated by a variety of kinases including MSK1, PKA and PKC isoforms.

LANCE Ultra Europium-anti-di/mono-methyl-Histone H3 Lysine 27 (H3K27me2-1) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 mono- and di-methylated at lysine 27 (H3K27me2-1). For use in LANCE® Ultra epigenetic writer and eraser assays.

ANGPTL4 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human ANGPTL4 Detection Kit is designed for detection and quantitation of human angiopoietin-like 4 protein in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

LANCE® Eu-W1024 labeled anti-GST antibody

Europium-labeled anti-GST antibody, for capturing GST-tagged proteins in LANCE® TR-FRET assays.

ErbB2 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human ErbB2 (HER2) Detection Kit is designed for detection and quantitation of human ErbB2 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
PerkinElmer

LANCE Eu-W1024 Anti-c-Myc

Europium-labeled anti-c-myc antibody, for capturing c-myc-tagged proteins in LANCE® TR-FRET assays.

GLP-1 (7-36) (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human GLP-1 (7-36) Detection Kit is designed for detection and quantitation of human GLP-1 (7-36) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
PerkinElmer

LANCE Eu-W1024 Anti-Human IgG

Europium-labeled anti-human IgG, for capturing antibodies in LANCE® TR-FRET assays.

IL-1β (mouse) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse IL-1β Detection Kit is designed for detection and quantitation of mouse interleukin 1β in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

Nidogen 1 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Nidogen 1 Detection Kit is designed for detection and quantitation of Nidogen 1 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

LANCE Ultra Europium-anti-tri-methyl-Histone H3 Lysine 27 (H3K27me3) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 tri-methylated at lysine 27 (H3K27me3). For use in LANCE® Ultra epigenetic writer and eraser assays.

VEGF (mouse) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse VEGF-A Detection Kit is designed for detection and quantitation of human CXCL1/GRO-α in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE Detection Kit

Aβ(1-40) (human) High Specificity LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Aβ(1-40) High Specificity Detection Kit is designed for detection and quantitation of human Aβ(1-40) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

ULight-anti-V5 (LANCE TR-FRET)

Anti-V5 tag antibody labeled with ULight™ dye. This ULight-labeled reagent can be used to capture V5-tagged proteins and peptides, and can be used in conjunction with LANCE® Europium-labeled reagents to create TR-FRET no-wash assays for protein-protein interaction, protein detection, and enzymatic studies.

CHO-P LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra CHO-P Detection Kit is designed for detection and quantitation of CHO proteins in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-STAT5 (Tyr694/699) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-STAT5 (Tyr694/699) kits are designed for the detection of STAT5 (phosphorylated at Y694/Y699) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
LANCE Assay Buffer, 2 mL

LANCE Assay Buffer

LANCE assay buffer is a buffer packaged in many of our LANCE Ultra biomarker detection assays.

Tau (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human TAU Detection Kit is designed for detection and quantitation of human Tau in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE Detection Kit

Albumin (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Albumin Detection Kit is designed for detection and quantitation of human albumin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

LANCE Ultra Europium-anti-methyl-Histone H3 Lysine 9 (H3K9me2)

Europium-labeled monoclonal antibody recognizing human Histone H3 di-methylated at lysine 9 (H3K9me2). For use in LANCE® Ultra epigenetic writer and eraser assays.
PerkinElmer

LANCE Ultra eIF2α Positive Control Lysate

Control lysates for LANCE Ultra TR-FRET cellular phospho-eIF2a detection assays.

"LANCE TR-FRET" 1-50363 产品与服务

每页