两家独立企业由此诞生
生命科学与诊断 & 分析和企业服务

了解更多
×
×

两家独立企业由此诞生

  • 生命科学与诊断业务

    专注于生命科学和诊断业务的高增长型公司，推动科学家和医疗卫生专业人士的研究、发现和检测，助力打造更健康的世界

  • 分析和企业服务业务

    我们专注于提供全方位的分析和企业服务解决方案，包括领先的OneSource实验室服务，加速您的科学研究进程

LANCE TR-FRET Assays | PerkinElmer
PerkinElmer

LANCE TR-FRET Assays

› ‹
OF

LANCE® (Lanthanide Chelate Excite) TR-FRET technology combines the benefits of Time Resolved Fluorescence (TRF) with those of Förster Resonance Energy Transfer (FRET) in a simple, highly-sensitive, and highly reproducible assay platform for detection, quantitation and screening in microplate format. We now also offer PerkinElmer Cisbio HTRF® assay technology. HTRF (Homogeneous Time Resolved Technology), allowing us to provide a wide range of assays kits in a homogenous TR-FRET technology.

The use of long-lifetime fluorescent lanthanide chelates, rather than other fluorophores, allows for a delay in measurement between excitation and emissions, which occur simultaneously in standard fluorometric assays. Furthermore, FRET detection utilizes energy donor and energy acceptor fluorophore labels in solution to create a no-wash detection assay. By combining the benefits of TRF and FRET, LANCE TR-FRET offers a multitude of benefits over standard fluorometric detection:

  • Homogeneous assay format (no wash steps, no separation steps)
  • Reduced background leading to better S:B
  • Large Stoke’s shift
  • Wider dynamic range
  • Extended signal stability
  • Increased sensitivity. Lower detection limits of 10 pg/mL compared to 100 pg/mL for standard fluorometric detection
  • Faster time to results

From biomarker detection assays, to cAMP/GPCR studies, to kinase and epigenetic assays, our LANCE® and LANCE Ultra TR-FRET assays are sure to save you time and hassle.

HTRF_84458_320x320f
HTRF_84458_320x320f

HTRF (Homogeneous Time Resolved Technology)

 

We now also offer PerkinElmer Cisbio HTRF® assay technology. Find out more about HTRF (Homogeneous Time Resolved Technology) and the products offered in this technology here.

Biochemical Assays
Biochemical Assays

Biochemical Assays

Biochemical assays remain an important tool for the drug discovery process. Whether you need a binding assay, kinase assay, other enzymatic assay, or would like to build your own assay utilizing our Toolbox reagents, LANCE has a variety of biochemical assays available.

推荐产品

LANCE Ultra Europium-anti-acetyl-Histone H3 Lysine 9 (H3K9ac), 10 µg LANCE Ultra Europium-anti-phospho-IkappaB-alpha (Ser32/36), 10 μg LANCE Ultra ULight™-IkappaB-alpha (Ser32/36) peptide, 0.5 nmole ULight anti-FLAG, 1 nmol LANCE Eu-W1024 Anti-Human IgG, 10µg LANCE Eu-W1024 Streptavidin, 50µg

建议内容

Serine/Threonine Kinase Profiling using the LANCE Ultra KinaSelect Ser/Thr Kit LANCE® Ultra Kinase Assay Selection Guide Biochemical Binding ADCC Assays Utilizing LANCE Toolbox Reagents for the Characterization of hIgGs a LANCE TR-FRET Immunoassay Conversion Guide LANCE TR-FRET protein-protein interaction assay development guide LANCE Protein: Protein Interaction Quick Start Guide
LANCE Ultra Biomarker
LANCE Ultra Biomarker

Assays for Biomarker Detection

Biomarker detection is key for advancing precision medicine. An array of biomarker detection kits are available utilizing our LANCE technologies in therapeutic areas including:
  • Angiogenesis
  • Animal Health
  • Autophagy
  • Biologics/Bioprocess
  • Cancer
  • Cardiovascular
  • Central nervous system
  • Inflammation
  • Metabolic

推荐产品

PD-L1 (human) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points PD-1 (human) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points TNFα (human) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points IL-1β (human) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points IFN-γ (human) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points

建议内容

High Throughput Quantitation of Cytokine Biomarkers using LANCE Ultra TR-FRET Assays Measuring PD-L1 and PD-1 Expression in Human Cells with LANCE Ultra TR-FRET Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET
Assays for Cell Signaling
Assays for Cell Signaling

Assays for Cell Signaling

Breakthrough discoveries in cell signaling and cell signal transduction are coming from laboratories that maximize high-quality results while minimizing hassles. And LANCE helps you do just that. With a wide selection of phospho- and total protein detection kits available, we are here to help you better understand the intracellular pathways of phosphorylated proteins.

推荐产品

Phospho-SLP-76 (Ser376) LANCE Ultra TR-FRET Cellular Detection Kit, 500 Assay Points Phospho-NFκB (Ser536) LANCE Ultra TR-FRET Detection Kit, 500 assay points Phospho-BTK (Tyr223) LANCE Ultra TR-FRET Detection Kit, 500 assay points Phospho-AKT 1/2/3 (Thr308) LANCE Ultra TR-FRET Cellular Detection Kit, 500 Assay Points Phospho-STAT3 (Tyr705) LANCE Ultra TR-FRET Cellular Detection Kit, 500 Assay Points Phospho-ERK 1/2 (Thr202/Tyr204) LANCE Ultra TR-FRET Cellular Detection Kit, 500 Assay Points

建议内容

LANCE Ultra TR-FRET Cellular Phosphorylation Quick Start Guide LANCE Ultra TR-FRET-based Detection and Modulation of Phosphorylated STAT3 Levels in Human Cells Optimization of LANCE Ultra Cell-based Phosphorylated ERK Assay Using the EnSight
OnPoint Services
OnPoint Services

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can label your assay components with LANCE donor and acceptor molecules. Or maybe you are you short on time and need an assay developed quickly? Custom LANCE assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.

推荐产品

Custom Assay Development Services Custom Reagent Labeling - LANCE
iStock
iStock

How can we help you?

When it comes to developing assays or troubleshooting issues, always know you’re never on your own. With over 30 years’ experience in defining, synthesizing and optimizing chelate and chelate-based bioanalytical assays PerkinElmer is here to help you succeed. Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.

FAS: Our global team of Field Application Scientists are hands-on subject matter experts in assay development. They are available to assist in both assay development and troubleshooting.

Application Support Knowledgebase (ASK): Need help at 1am? We’ve got it covered. The Application Support Knowledgebase (ASK) contains detailed information on assay development, protocols, tips, FAQs, citations, troubleshooting and more. Accessible 24 hours a day, 7 days a week on our website. https://www.perkinelmer.com/LANCE/ASK.

产品与服务 (262)
Filters:
相关分类

筛选条件

全部展开 全部折叠
检测目标
  • Adiponectin (2)
  • Akt1 (1)
  • Akt1/2/3 (2)
  • Albumin (2)
  • AMPKα (1)
  • ANGPT1 (2)
  • ANGPTL3 (2)
  • ANGPTL4 (2)
  • ApoC3 (2)
  • ApoE (2)
  • Bcl2 (2)
  • BTK (2)
  • c-Myc (2)
  • cAMP (1)
  • CCL2 (3)
  • CCL3 (2)
  • CCL4 (2)
  • COL1A1 (2)
  • CRP (2)
  • CXCL1 (2)
  • CXCL10 (2)
  • CXCL9 (2)
  • E-Cadherin (2)
  • EGF (3)
  • EGFR (3)
  • eIF2α (1)
  • ERK1/2 (2)
  • Fibronectin (2)
  • Frataxin (2)
  • GAD67 (2)
  • hCG (2)
  • IFN-λ2 (2)
  • IFNα (2)
  • IFNγ (4)
  • IGF1 (2)
  • IgG (6)
  • IL-11 (1)
  • IL-23A (2)
  • IL11 (1)
  • IL12 (2)
  • IL17A (2)
  • IL1α (2)
  • IL1β (3)
  • IL2 (2)
  • IL5 (1)
  • IL6 (5)
  • IL8 (2)
  • Insulin (2)
  • Kappa light chain (2)
  • LCK (1)
  • MIP1α (2)
  • MMP10 (2)
  • MMP3 (2)
  • MMP7 (2)
  • NFκB (2)
  • Oncostatin M (2)
  • p53 (2)
  • PAI-1 (2)
  • PCSK9 (1)
  • PD-1 (2)
  • PD-L1 (2)
  • PIP Collagen (2)
  • Residual DNA (2)
  • SLP-76 (2)
  • SMAD2 (1)
  • SMAD3 (2)
  • Src (2)
  • STAT1 (3)
  • STAT3 (2)
  • STAT5 (1)
  • TBK1 (2)
  • TNFα (4)
  • tPA (1)
  • TSH (2)
  • VEGF-A (4)
  • Zap70 (1)
应用
  • Biologics/Bioprocess (11)
  • cAMP (1)
  • Enzymatic (6)
  • Epigenetics (11)
  • Kinase (75)
  • Protein Analysis & Detection (143)
  • Protein-protein interactions (18)
检测目标类
  • Antibody (8)
  • Cytokine (52)
  • DNA (2)
  • Histone (1)
  • Hormone (6)
  • Peptide (2)
  • Phosphoprotein (33)
  • Protein (78)
治疗领域
  • Autoimmunity (30)
  • Cardiovascular (16)
  • Immuno-oncology (18)
  • Inflammation (52)
  • Metabolic (44)
  • Neuroscience (27)
  • Oncology (74)
  • Respiratory (9)
  • Virology (1)
产品品牌名称
  • LANCE (22)
  • LANCE Ultra (235)
  • LANCE SureLight (7)
检测方法
  • Time-Resolved Fluorescence (TRF) (228)
  • TR-FRET (228)
分子修饰
  • Acetylation (4)
  • Methylation (8)
  • Phosphorylation (39)

排序方式

1-25 的 262 产品与服务

  • Jump to:
技术资源 (242)
Filters:

筛选条件

全部展开 全部折叠
活动

1-1 的 1 技术资源