Targeted sequencing enables scientists to focus on sequences or regions of interest. Because less genomic sequence is being interrogated, only target regions of interest are sequenced. This means that by focusing the sequencing real estate of the flow cell to only regions of interest, genomic sequences can be enriched anywhere from 100 to a 1 million times compared to DNA sequencing. This is critical for some applications where the typical 10x or 100x coverage obtained by DNA sequencing or even large exome-sequencing may not be sufficient, such as for the detection of low-level somatic variants that may require 3,000x coverage or more to be detected at a frequency of 5% or less.
PerkinElmer offers a complete line of NEXTFLEX® library preparation kits to meet your targeted sequencing needs.
For research use only. Not for use in diagnostic procedures.