PerkinElmer
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Phospho-Syk (Tyr352) AlphaLISA SureFire Ultra Detection Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ phospho-Syk assay is a sandwich immunoassay for quantitative detection of Syk (phosphorylated on Tyr352) in cellular lysates using Alpha Technology.

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For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
ALSU-PSYK-B-HV
100 Assay Points
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ALSU-PSYK-B500
500 Assay Points
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ALSU-PSYK-B10K
10,000 Assay Points
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ALSU-PSYK-B50K
50,000 Assay Points
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详情 信息

Formats:

  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 µL reaction volume.

In the AlphaLISA SureFire Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

AlphaLISA SureFire Ultra™ kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

规格

检测目标 SYK
检测目标类 Phosphoprotein
自动化兼容 Yes
检测方法 Alpha
产品品牌名称 AlphaLISA SureFire Ultra
运输条件 蓝冰
产品尺寸 10,000 Assay Points