PerkinElmer
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IFN-γ (ovine) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for ovine interferon gamma (IFNγ) enables the quantitative determination of ovine IFNγ in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
AL560HV
100 Assay Points
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AL560C
500 Assay Points
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AL560F
5,000 Assay Points
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Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Interferon gamma becomes one of important biomarkers for determining infectious diseases in human and in domestic animals. It is critical for innate and adaptive immunity against viral, bacterial and protozoan infections. In domestic animal species, IFNγ is associated with autoinflammatory and autoimmune diseases and inhibits viral replication and bacterial infections. Ovine IFNγ has been identified as a biomarker for vaccine responses, viral, and bacterial infection. This kit is designed to detect oIFNγ in serum, plasma, and cell culture supernatants.

规格

检测目标 IFNγ
检测目标类 Cytokine
自动化兼容 Yes
检测方法 Alpha
实验类型 In vitro
产品品牌名称 AlphaLISA
运输条件 蓝冰
Target Species Ovine
治疗领域 Inflammation
产品尺寸 100 Assay Points
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AlphaLISA Ovine IFNγ Detection kit AL560

This manual explains how to run the AlphaLISA no-wash ovine IFN? detection assay.

PDF 597 KB