PerkinElmer
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CHO HCP LANCE Ultra TR-FRET Detection Kit, 10,000 assay points

The LANCE® Ultra CHO HCP Detection Kit is designed for detection and quantitation of CHO host cell proteins in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
TRF1301C
500 Assay Points
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TRF1301M
10,000 Assay Points
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详情 信息

The LANCE Ultra CHO HCP Detection Kit is designed for detection and quantitation of CHO host cell proteins in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

The 500 point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample). The 10,000 point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample).

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

CHO (Chinese Hamster Ovary) cells are commonly utilized as an expression system for the production of human recombinant proteins at industrial scale. However, since very frequently these proteins are intended to be used as therapeuticals for humans, they must be subsequently purified to remove undesired contaminants that could elicit toxic or immunological reactions. Therefore, there is a current need to quantitate and trace these unwanted host cell proteins in protein preparations with excellent sensitivity and robustness, while maintaining high throughput capabilities.

规格

检测目标 CHO HCP
检测目标类 Protein
自动化兼容 Yes
实验类型 In vitro
产品品牌名称 LANCE Ultra
运输条件 蓝冰
Target Species CHO
治疗领域 Biologics/Bioprocess
产品尺寸 10,000 Assay Points