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Opera Phenix Plus High-Content Screening System

The Opera Phenix® Plus High-Content Screening System is the premier confocal solution for today's most demanding high content applications. Drawing on over a decade of experience with the industry-leading Opera® and Opera® Phenix system, the Opera Phenix Plus is designed for high-throughput high-content assays, phenotypic screening, assays using complex disease models, such as live cells, primary cells and microtissues, and fast-response assays, such as Ca2+ flux.

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Part Number HH14001000
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Deeper Insights From Your Most Demanding Assays

The Opera Phenix Plus high-content screening system’s innovative optical design lets you generate richer information through extremely sensitive confocal imaging and at higher throughput than ever through simultaneous acquisition. Because spectral crosstalk is reduced to a minimum, it delivers speed without compromising sensitivity. With proven automated water immersion lenses, you’ll achieve higher throughput and richer content, making it the ideal high-content screening system for discriminating phenotypes and studying complex disease models. And with its liquid handling option and fast imaging frame rate, you can tackle fast-response assays such as calcium flux or cardiomyocyte beating.

Speed or Sensitivity – No Compromise

Proprietary Synchrony Optics combine a microlens enhanced Nipkow spinning disk with dual view confocal optics to separate fluorescence excitation and emission during simultaneous acquisition minimizing spectral crosstalk – for greater speed and higher sensitivity.

  • Up to four large format sCMOS cameras with simultaneous acquisition in up to four channels, so you can generate images of exceptional quality - faster than ever
  • Custom-designed high NA water immersion objectives to capture more photons and provide high image resolution even in thick samples
  • State-of-the-art sCMOS image sensors deliver low signal-to-background noise, a wide dynamic range and high resolution – ideal for sensitive and quantitative measurements at short exposure times
  • Spinning microlens disk with increased pinhole-to-pinhole distance to further reduce out-of-focus noise in thick samples such as microtissues
  • Spinning disk optics and precise synchronization of the excitation laser and camera to minimize phototoxicity and bleaching for live cell assays
  • Widefield or confocal imaging modes depending on your assay requirements
  • Fast laser-based autofocus to ensure sharp confocal images from every field
  • Modular design to adapt the system to your changing application needs – choose from single, dual or four camera configuration with four solid state lasers or a five laser system optimized for FRET applications

Capture Fast Cellular Responses

The system’s imaging frame rate of up to 100fps is well-suited to assays that measure the beat-rate of cultured cardiomyocytes, as often used in cardiotoxicity studies. And the fast imaging frame rate, together with the dispense and read capabilities of the optional pipettor module, enables fast response assays in which cell responses occur within milliseconds to seconds – notably calcium flux assays.

  • Tip-based pipettor compatible with PerkinElmer 20µl and 200µl tips, 1-200µl in 1µl increments
  • Compound addition from one compound plate and one reservoir, up to 2 additions per well
  • Compatible with 96- and 384-well sample plates
  • Environmental control - temperature, CO2, and humidity
  • Compatible with automated exchange of sample plate, tip racks and compound plate
  • Liquid handling module is compatible with brightfield imaging

Opera Phenix Plus Configurations

Opera Phenix Plus Configurations

It All Comes Together in Harmony® – Acquisition to Analysis Made Easy

  • Intuitive workflow user-interface guides you through the entire process, from acquisition to analysis and evaluation
  • Templates for set-up of acquisition channels and parameters to get you productive right away
  • Ready-made solutions for common image analysis tasks so you can simply select and go
  • Image analysis building blocks that allow you to create, configure, and customize your own high content analysis applications
  • Advanced analysis features such as texture and STAR morphology analysis for detailed descriptions of cellular morphology and robust differentiation of cellular phenotypes
  • Data management for automatic storage of analysis results and metadata, such as assay layout, instrument, settings, plus user-defined keywords, and annotations
Steve Bagley
Loic Dupre

Your Total Solution for High Content Screening

  • Get better results from your Opera Phenix Plus system with our microplates for high content screening, such as the PhenoPlate 384-well microplates designed for optimal performance in high content imaging applications and our CellCarrier Spheroid ULA plates for imaging 3D models
  • Improve throughput and productivity, and reduce variability and reagent costs by automating your Opera Phenix system, or benefit from automated workflows with the explorer G3 workstations for cell painting, 3D cell culture or phenotypic screening
  • Export results automatically into Signals Image Artist Image Analysis and Management platform, so you can access, re-analyze, store, and share all your cell image data from Opera Phenix Plus and other HCS systems
  • Use with Signals VitroVivo to perform screening data analysis and validation, QC analyses, calculate reliable normalization, multivariate hit stratification, dose response curves, and drug response profiling

Resources, Events & More
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Application Note

Analyzing ERK Signal Transduction in Live Cells Using a FRET-Based Biosensor

Extracellular signal-regulated kinase (ERK) is a key component in the regulation of embryogenesis, cell differentiation, cell proliferation, and cell death. The ERK signaling pathway is altered in various cancer types and is frequently investigated as a target for therapeutic intervention. This appl ...

Automated Single Cell Tracking using the Operetta System: Analyzing Chemokinesis of Cancer Cells

In this application note, we describe a high-content screening application for analyzing the migration of non-small cell lung cancer cells in a live cell assay. Using the Operetta® high-content imaging system and digital phase contrast imaging, we tracked migrating cancer cells using automated singl ...

Cell Painting for Phenotypic Screening

Cell Painting is a powerful high-content screening method which combines cell and computational biology to unravel cells’ responses and gain a deeper understanding of the effects of chemical and genetic perturbagens.

However, implementation of cell painting is not without its challenges - f ...

Distinguishing Cell Types by Phenotypic Profiling of the Nucleus

The promise of high-content screening is the acceleration of discovery by extracting as much relevant information as possible from cells. Nevertheless, a large percentage of high-content screens analyze only a small number of image-based properties. As a result, valuable information from precious c ...

Fast Kinetic Calcium Flux Imaging Using the Opera Phenix Plus High-Content Screening System

Dysregulation of GPCR signaling, particularly calcium signaling, has been implicated in different diseases, e.g. cancer and Alzheimer’s disease. Currently about 34% of all FDA approved drugs target GPCRs indicating their importance for drug discovery, and in vitro cellular GPCR assays are importa ...

PDF 435 KB
Imaging Bile Canaliculi in 3D Liver Microtissues

Analyzing transport of biliary metabolites is essential to predict pharmacokinetics and hepatotoxicity during drug development. A functional impairment of hepatobilary transporters, such as bile salt export pump (BSEP) and multidrug resistance-associated protein 2 (MRP-2), is strongly associated wit ...

Label-Free Analysis of Cardiomyocyte Beating using the Opera Phenix Plus System

Drug-induced cardiotoxicity is a frequent cause of drug attrition during drug development and can lead to the withdrawal of drugs from the market, so non-invasive approaches that allow quick and reliable assessment of the cardiotoxic potential of a compound are key to improve drug safety.

I ...

Measuring FRET using the Opera Phenix High-Content Screening System

Fundamental processes in living cells, such as apoptosis and signal transduction are controlled by proteins, often acting in concert with other protein partners through protein-protein interactions (PPIs). Inappropriate protein-protein recognition can fundamentally contribute to many diseases, inclu ...

Phenotypic Analysis of Hypertrophy in Human iPSC-Derived Cardiomyocytes

Learn how a phenotypic screening assay to study time-dependent effects of endothelin-1-induced hypertrophy was set up using human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes. Learn how: The Opera Phenix system has been applied in the field of neurodegenerative diseases. In this assa ...



5 Top Tips for a Successful High-content Screening Assay with a 3D Cell Model System

Whether you’re familiar with high-content screening and are looking to exploit the increased physiological relevance of complex 3D cell models, or you want to take your analysis of 3D cell models to the next level, migrating from simple plate-reader assays to a high-content approach, you’ll need the ...

Bone Morphogenetic Protein 8B - A Promising Therapeutic Avenue for NASH Treatment

The incidence and prevalence of non-alcoholic fatty liver disease (NAFLD), which is characterized by excessive accumulation of lipids within the liver, is high and progressively rising due to increasing obesity and metabolic syndrome prevalence. For some patients, NAFLD can progress to non-alcoho ...

Cell Painting: Your Questions Answered

With powerful potential for phenotypic drug discovery, cell painting is being rapidly adopted by leading scientists across the globe. However, implementing cell painting assays can present some challenges.

To help you avoid some of the common pitfalls, we’ve collated answers from our expert ...

PDF 470 KB
Prolonging ß Cell Proliferation with a 3D Culture System

Within diabetes research, an evolving area of interest is the identification and development of an efficacious treatment for ß cell loss. The following literature review discusses the use of a Disque platform for evaluating the functionality of multiple drug candidates.

The Why and How of Live-Cell Imaging for Research and Drug Discovery

Live-cell imaging, the study of living cells using microscopy, has become a requisite technology in many fields of biomedical research, such as cell biology, developmental biology and cancer research. Also, in drug discovery, researchers adopt live-cell imaging as they look for a more detailed un ...

hESC-Derived Cardiomyocyte Screening Platform Identifies Novel Inhibitors of SARS-Cov-2 Viral Entry

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the virus that causes COVID-19, the respiratory illness responsible for the COVID-19 pandemic. Studies have shown that patients infected with SARS-CoV-2 with cardiovascular comorbidities are more susceptible to severe infection and t ...



3D Cell Culture Solutions Brochure

Download our brochure to learn how our solutions help you to grow, detect, and analyze 3D cells.

CRISPR Workflow Solutions

CRISPR is transforming research by enabling the modulation of hundreds or thousands of genes in a single experiment to identify genetic pathways, cellular processes, novel therapeutic targets, and to genetically profile existing or potential therapeutics.. This solution guide will help you confid ...

Complete Solutions for NASH Research

When Non-alcoholic fatty liver disease (NAFLD) progresses to nonalcoholic steatohepatitis (NASH), with inflammation and damage to liver cells it can cause fibrosis, cirrhosis and liver failure or cancer. Despite the prevalence of NAFLD and NASH rising, their remains no approved treatments. But st ...

Drug Discovery Screening Solutions Brochure

Find out about our range of integrated solutions for drug discovery screening in this e-brochure.

Our screening solutions for high-throughput screening, phenotypic screening and data analysis help to streamline drug discovery workflows in labs across the globe. Our portfolio includes automat ...

Oncology Research Solutions

Every day, researchers are furthering our understanding of cancer biology, uncovering new mechanisms driving the disease. These discoveries provide new targeting options that ultimately drive the next generation of therapeutics. With this guide, discover a diverse range of solutions designed to ...

Opera Phenix Plus High-Content Screening System Brochure

The Opera Phenix® Plus High-Content Screening System is the premier confocal solution for today's most demanding high content applications.


Case Study

B Cell Selection and Therapeutic Antibody Characterization Using the Operetta High Content Imaging System

This case study illustrates how PerkinElmer’s high-content imaging solutions are enabling therapeutic antibody development. Two steps in the workflow - the clonal B cell selection and the functional antibody characterization - rely on high-content screening using the Operetta® system. ...

Improving the Throughput of a Neuroprotection Assay Using the Opera Phenix High-Content Screening System

This case study shows how a previously-described neuroprotection assay was easily and directly transferred to the Opera Phenix® high-content screening system, with a 4-fold decrease in acquisition time. In the assay, primary rat neurons are co-cultured on top of rat-derived astrocytes. ...

Re-Tooling Anti-Microbial Research for the 21st Century

Antimicrobial resistance (AMR) is one of today’s major global public health challenges. Recently, there has been renewed investment in the discovery of novel antimicrobials to urgently address the growing number of drug-resistant infections. This case study describes how Professor Gordon Dougan a ...

PDF 997 KB


Adding Depth: 3-D Cell Culture

Download this booklet from The Scientist and PerkinElmer to learn about how the third dimension affects cell behavior, the similarities and differences between 2-D and 3-D culture, common 3-D culture models, and how to image and analyze 3-D culture models.

Cellular Analysis in 3D

While 3D cell culture provides unprecedented opportunities for both increased physiological relevance and analysis using a high-content approach, it is also more complex than traditional 2D cell culture. This booklet, from Biocompare and PerkinElmer, will unravel some of the complexities often encou ...

High-Content Analysis: Advancing Your Knowledge to Help Combat Infectious Disease

Infectious diseases remain a major burden to human health. The increased globalization of modern society that facilitates the spread of infectious diseases, and phenomena such as anti-microbial resistance, underscore the importance of the development of new preventative and therapeutic approaches ...

Live-cell Imaging eBook

Live-cell imaging has evolved from allowing observation of large-scale changes to capturing subtle changes in dynamic cellular processes. Today, live-cell imaging coupled with high-content analysis enables researchers to extract quantitative data in real-time, facilitating new insights in basic l ...



Five Top Tips for a Successful High-Content Screening Assay with a 3D Cell Model System

Whether you’re familiar with high-content screening, or a newcomer, you’ll need the right tools and strategies to overcome the challenges of using complex 3D cell models in such an assay. For example, growing consistent, reproducible 3D cultures can be problematic and imaging large, thick cell sampl ...


Publication Review

Morphological Profiling of Human T and NK Lymphocytes by High-Content Imaging

Advances in high-content cellular imaging (HCI) and analysis have enabled researchers to obtain rich, quantitative data from cells and identify biologically relevant similarities and differences in their morphological profiles. Morphological profiling has been employed in cancer and toxicology re ...

Publications for High-Content Screening

Publications in which PerkinElmer high-content analysis and screening systems are used number more than 1000 between 2010 and 2019. Here we feature some of the more recent publications, focussing on Operetta CLS and Opera Phenix, and categorized by application or research area.


Technical Note

3D Volumetric Analysis of Luminal Spaces Inside Cysts or Organoids

High-content assays using 3D objects such as cysts or organoids can be challenging from the perspectives of both image acquisition and image analysis. In this technical note we describe how to image and analyze epithelial Madin-Darby canine kidney (MDCK) cysts in 3D on the Operetta CLS

3D Volumetric and Zonal Analysis of Solid Spheroids

Multicellular 3D “oids” (tumoroids, spheroids, organoids) have the potential to better predict the effects of drug candidates during preclinical screening. However, compared to 2D cell monolayers, assays using 3D model systems are more challenging.

In this technical note we describe how to ...

Clearing Strategies for 3D Spheroids

In drug discovery programs, multicellular spheroids have emerged as powerful tools to bridge the gap between in vitro cell culture models and in vivo tissues. However, one of the greatest challenges in higher throughput 3D imaging is the acquisition of images of solid spheroids, owing to the redu ...

How to Perform Successful Long Term Live Cell Imaging in a High-Content Analysis System

Live cell imaging has gained importance within drug discovery over recent times, as researchers look for more meaningful insights into cellular behavior and function. However, setting up live cell experiments can be challenging, as temperature, CO2 and evaporation need to be controlled ...

How to Perform Successful Long Term Live Cell Imaging in a High-Content Analysis System

Download our technical note to find out how you can overcome some of the challenges associated with long-term live cell imaging. Learn how you can perform successful five-day live cell imaging on Operetta CLS™ and Opera Phenix™ high-content systems, avoid phototoxicity with gentle digital phase cont ...

Improved High-Content Imaging of Tissue Sections

Automated image acquisition and analysis of tissue sections can be challenging owing to the inherent height variations throughout each specimen, and this can hinder their use in screening applications.

In this technical note we show how PreciScan intelligent image acquisit ...

Opera Phenix High-Content Screening System Technical Performance: Speed

The Opera Phenix™ high-content screening system’s state of the art hardware, combined with Harmony® imaging and analysis software, improves acquisition and analysis speed, enabling users to perform highly multi-parametric phenotypic screens effectively. In this technical note, learn how the Opera P ...

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Opera Phenix High-Content Screening System: Crosstalk Suppression

Simultaneous multi-color imaging is a technology commonly used to increase the speed of high content screening systems. However, one of the main problems arising from this approach is spectral crosstalk which can limit assay sensitivity. To overcome this challenge, we have developed an innovative ...

Opera Phenix High-Content Screening System: Improved 3D Imaging

Spinning disk confocal microscopy is a common tool for live cell microscopy and reduces background fluorescence from out of focus planes. However, for 3D imaging, confocal image quality can be limited by a phenomenon known as ‘pinhole crosstalk’ which can lead to blurred images in conventional sp ...

The Benefits of Water Immersion Lenses for High-Content Screening

The key parameters in high-content imaging - speed, sensitivity (or intensity) and resolution - cannot be optimized independently as altering one of them influences the others. For example, increasing the resolution by choosing a higher magnification requires imaging of more fields to maintain th ...


White Paper

Advancing Neurological Treatments at Synaptic Speed

There is a huge unmet need for the development of treatments that target the underlying causes of neurodegenerative diseases. To address the high failure rates and low investment returns of traditional neuroscience R&D approaches, companies are increasingly adopting alternative R&D business model ...

PDF 507 KB
Artificial Intelligence, Machine Learning and Deep Learning: Applications in Cellular Imaging for Improved Drug Discovery Productivity

There has been a lot of buzz around artificial intelligence, machine learning and deep learning. Is the reality living up to the hype?

In the world of cellular imaging and its application to drug discovery, there is evidence of real progress against some of the critical challenges facing s ...

Cell Painting - A Cellular Imaging and Machine Learning Approach to Drug Discovery

Phenotypic Drug Discovery Re-Imagined

Cell Painting is a phenotypic screening method and a powerful application of high-content screening technology which combines cell and computational biology to elucidate the behavior of cells under the influence of perturbagens, such as chemical compoun ...

PDF 502 KB
High Content Screening in Three Dimensions

Researchers are increasingly looking to 3D cell cultures, microtissues, and organoids to bridge the gap between 2D cell cultures and in vivo animal models. This whitepaper documents a streamlined procedure for getting the most information, as quickly as possible, using solutions from PerkinElmer.

High-Throughput Approaches to Overcome Antimicrobial Resistance

A team of researchers exploits high-content imaging to phenotype the effects of antimicrobial exposure on individual bacteria cells and screen for novel alternatives to existing antimicrobials. Projects include the development of polyclonal antibody serum, monoclonal antibodies, and functional as ...

Leveraging the Microbiome to Strengthen Immune Therapeutic Response

Researchers are striving to determine where and when relevant bacteria play a role in cancer progression and whether those interactions are localized to the gastrointestinal tract or occur in other tissues before malignancy. Understanding the role bacteria play, whether it be oncogenesis, tumor p ...

PDF 502 KB
Phenotypic Drug Discovery with High Content Screening

Today's drug discovery strategies require candidate compounds to fail early and cheaply in the discovery stage, rather than late and expensively in the clinical phase. Testing compounds early in physiologically relevant model systems and leveraging rich information from image-based screens are wa ...

PDF 903 KB
Postbiotics: A Paradigm Shift in Microbiome Research

At the University of Milano-Bicocca, researchers in the laboratory of Professor Francesco Peri have been investigating the impact of molecules produced by microbiota metabolism, known as postbiotics, on the human host. The team has been using using high-content imaging and AlphaLISA immunoassays ...

PDF 988 KB
Therapeutic Targeting of the Tumor Microenvironment

Tumor cell function is critically dependent on the local microenvironment and needs to be considered when devising therapeutic strategies to overcome resistance. In this white paper, we discuss how 3D models will help close the gap between what is seen in the preclinical setting and translation i ...

PDF 286 KB