Opera Phenix® Plus高内涵筛选系统是用于当今最具挑战的高内涵应用的高级共聚焦解决方案。在业内领先的Opera®和Opera® Phenix系统十多年应用经验的基础上，Opera Phenix Plus系统设计用于高通量高内涵试验、表型筛选、使用复杂疾病模型的试验（如活细胞、原代细胞和微组织）以及快速动力学试验（如Ca2+流试验）。
false falseFor research use only, not for use in diagnostic procedures.
更深入地了解高要求试验
Opera Phenix Plus高内涵筛选系统采用创新光学设计，通过极其灵敏的共聚焦成像与同步采集，在前所未有的高通量下提供丰富的信息。由于最大化减小了染料间串扰，该系统能够同时确保高速度与高灵敏度。凭借久经考验的自动化水镜系统，该高内涵筛选系统能够提供高通量与丰富的信息，是用于区分表型和研究复杂疾病模型的理想之选。此外，由于具备液体处理模块和高速成像功能，该系统可处理钙流与心肌细胞跳动等快速动力学试验。
同时确保速度与灵敏度
专有Synchrony™ Optics将微透镜增强型Nipkow转盘与双光路共聚焦光学元件相结合，在同步采集的过程中分离荧光激发与发射光，最大化减少染料间串扰-提供更高的速度与灵敏度。
捕获快速细胞反应
该系统的成像帧频最高可达100fps，适用于测定心肌细胞收缩频率的试验（常见于心脏毒性研究）。此外，凭借高成像帧频与选配移液器模块，该系统能够轻松完成细胞在几毫秒到几秒之间发生的快速反应的检测试验，尤其是钙流试验。
Harmony®集成并简化了从采集到分析所有流程
高内涵筛选的整体解决方案
| 产品品牌名称 | Opera Phenix Plus |
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传染病始终是对类健康的一大负担。现代社会的全球化趋势促进了传染病的传播以及耐药性等现象的出现，使新预防和治疗方法的开发变得越来越重要。请下载本小册子，了解如何通过高内涵成像与分析完成可适应于一系列病原体的高通量功能与表型分析；请阅读描述高内涵分析对埃博拉、寨卡、肺结核、李斯特菌和疟疾等疾病研究的贡献的一系列特色出版物，寻找利用高内涵方法研究寄生、病毒与细菌性疾病的案例。
The culture and analysis of organoids presents complex challenges, especially when it comes to scaling up for drug discovery purposes. This guide will help you navigate these challenges and find innovative solutions to enhance organoid research.
Glioblastoma is the most common type of malignant brain tumor in adults. It is usually very aggressive, with less than 10% of patients expected to survive five years following diagnosis. Traditional treatment strategies are rarely curative, emphasizing the urgent need to discover more effective ther ...
Advances in high-content cellular imaging (HCI) and analysis have enabled researchers to obtain rich, quantitative data from cells and identify biologically relevant similarities and differences in their morphological profiles. Morphological profiling has been employed in cancer and toxicology resea ...
Publications in which PerkinElmer high-content analysis and screening systems are used number more than 1000 between 2010 and 2019. Here we feature some of the more recent publications, focussing on Operetta CLS and Opera Phenix, and categorized by application or research area.
Macrophages are the primary host cells for Mycobacterium tuberculosis (Mtb) during its intracellular survival in humans, and it has recently been suggested that autophagy plays a role in the response of host macrophages to Mtb infection. This present study details how researchers at The Francis Cric ...
从高通量到高内涵药物筛选完全解决方案
The Opera Phenix® Plus High-Content Screening System is the premier confocal solution for today's most demanding high content applications.
Every day, researchers are furthering our understanding of cancer biology, uncovering new mechanisms driving the disease. These discoveries provide new targeting options that ultimately drive the next generation of therapeutics. With this guide, discover a diverse range of solutions designed to acce ...
The need for better profiling is clear, and technological advances are making it possible to optimize clinical trials and ultimately develop precise therapies. Read our precision medicine research ebook and get the clarity you need in your research to identify the right target – for the right indivi ...
CRISPR is transforming research by enabling the modulation of hundreds or thousands of genes in a single experiment to identify genetic pathways, cellular processes, novel therapeutic targets, and to genetically profile existing or potential therapeutics.. This solution guide will help you confident ...
Truly understanding infectious diseases is the first step toward containment and eradication. From biology to breakthrough, our diverse infectious disease product portfolio helps researchers make critical discoveries through genomic, protein, cell, and in vivo analysis.
Find out about our range of integrated solutions for drug discovery screening in this e-brochure. Our screening solutions for high-throughput screening, phenotypic screening and data analysis help to streamline drug discovery workflows in labs across the globe. Our portfolio includes automated liqui ...
When Non-alcoholic fatty liver disease (NAFLD) progresses to nonalcoholic steatohepatitis (NASH), with inflammation and damage to liver cells it can cause fibrosis, cirrhosis and liver failure or cancer. Despite the prevalence of NAFLD and NASH rising, their remains no approved treatments. But studi ...
在这个实验中，我们建立了一种快速稳定的基于FRET的高内涵检测...
细胞外信号调节激酶(ERK)是胚胎形成、细胞分化、细胞增殖与细胞死亡的关键调节成分。ERK信号通路依癌细胞类型的变化而变化，经常作为治疗性干预的靶标而被研究。该应用文献描述如何利用Operetta CLS™高内涵分析系统开展研究ERK信号的活细胞FRET试验。基于FRET的生物传感器的优化设计、Operetta CLS系统的高质量成像以及易于使用的Harmony®图像分析软件共同确保了高内涵试验的可靠性。
高内涵筛选的优势在于通过从细胞中提取尽可能多的相关信息来加速药物研发的过程。大部分高内涵筛选仅分析少量基于图像的属性，导致从细胞和疾病模型中获得的有价值的信息未被充分利用。为促进细胞分割，几乎所有筛选方法均要求细胞核染色（如Hoechst），因此细胞核表型分析可为试验提供新角度，同时还不会产生额外成本。
学习如何利用人类诱导多能干细胞(hiPSC)诱导分化的心肌细胞建立表型筛选试验，以研究内皮素-1诱导的细胞肥大的时间依赖性。学习：Opera Phenix在神经退行性疾病领域的应用。试验使用的Opera Phenix系统比之前的Opera®系统快4倍。主要的神经元形态通过Harmony软件直接分析。认真优化试验可在不影响试验性能的情况下提高通量并最大程度减少数据负载。
本应用文献描述如何利用高内涵筛选在活细胞试验中分析非小细胞肺癌细胞的迁移。凭借Operetta®高内涵成像系统与明场无标记成像，我们利用Harmony®高内涵成像和分析软件的自动化单细胞追踪功能追踪癌细胞的迁移。
Drug-induced cardiotoxicity is a frequent cause of drug attrition during drug development and can lead to the withdrawal of drugs from the market, so non-invasive approaches that allow quick and reliable assessment of the cardiotoxic potential of a compound are key to improve drug safety. In this ap ...
Cell Painting is a powerful high-content screening method which combines cell and computational biology to unravel cells’ responses and gain a deeper understanding of the effects of chemical and genetic perturbagens. However, implementation of cell painting is not without its challenges - from choos ...
Analyzing transport of biliary metabolites is essential to predict pharmacokinetics and hepatotoxicity during drug development. A functional impairment of hepatobilary transporters, such as bile salt export pump (BSEP) and multidrug resistance-associated protein 2 (MRP-2), is strongly associated wit ...
Combining CRISPR gene editing and cell painting provides researchers with the ability to correlate genotypes with phenotypes and holds the promise to help understand gene functions in more detail and at greater scale. This application note demonstrates a harmonized workflow for morphological profili ...
Dysregulation of GPCR signaling, particularly calcium signaling, has been implicated in different diseases, e.g. cancer and Alzheimer’s disease. Currently about 34% of all FDA approved drugs target GPCRs indicating their importance for drug discovery, and in vitro cellular GPCR assays are important ...
在药物研发项目中，多细胞微球已成为缩小体外细胞培养模型与体内组织间差异的有力工具，但由于透光量下降，采集固态球状体图像成为了高通量3D成像面临的一大困难。一种解决方案是使用透明化技术，通过清除脂质和蛋白质分子增大球状体内的透光深度。本技术说明对比了用于3D微球的不同透明化方案，量化了透明化程度和形态变化，还描述了如何将3D球状体中的成像深度增大四倍。
多细胞3D“oids”（囊肿样、球状体、细胞器）能够更好地在临床前筛选中预测候选药物的影响，但相比于使用2D细胞单层，使用3D模型系统的试验更难以开展。
本技术说明描述如何利用高内涵筛选系统以及成像和分析软件对球状体进行3D成像与分析。
本技术说明描述：
请下载本技术说明，了解如何克服与长期活细胞成像相关的若干困难，学习如何利用Operetta CLS™和Opera Phenix™高内涵系统连续五天进行活细胞成像、如何通过明场无标记成像技术避免光毒性以及如何在不进行荧光标记的情况下分析单细胞的生长和形态。
利用囊肿或空腔细胞器等3D形态的高内涵分析在图像采集与图像分析方面存在困难。本技术说明描述如何利用Operetta CLS™高内涵分析系统对马丁达比狗肾(MDCK)囊肿进行3D成像与分析。
本技术说明描述：
由于样品本身高度差异大，因而难以对组织切片进行自动化图像采集和分析，这限制了组织切片在筛选分析中的应用。
本技术说明描述了PreciScan™智能图像采集功能如何在大幅减小数据量和缩短采集时间的同时，通过克服样品高度变化或组织切片在载玻片上的位置变化等因素造成的问题来生成准确的高分辨率图像。
转盘共聚焦显微镜是活细胞显微镜的常用技术方案，能够减少非焦面产生的本底荧光，但在3D成像中，导致常规转盘系统图像模糊的“针孔串扰”现象会限制共聚焦图像的质量。在Opera Phenix™高内涵筛选系统中，共聚焦扫描转盘的针孔间距较大，减少了针孔串扰，改善了3D图像质量。请阅读技术说明，了解更多信息。
在近几年，随着研究人员寻求对细胞行为和功能的更深入了解，活细胞成像在药物研发领域变得越来越重要，但活细胞试验难度较高，因为为确保最佳的细胞生长条件，需对温度、CO2和蒸发速度进行控制。本技术说明描述了：
高内涵成像中的关键参数（速度、灵敏度（或光强度）与分辨率）无法单独优化，因为改变其中任何一个参数均会影响其它参数。例如，通过选择较大的放大倍率提高分辨率时，为保持细胞量不变，需提高成像视野数，但这会降低速度；或通过延长曝光时间提高荧光强度时，速度也会下降。这些问题可以克服。本技术说明解释了正确选择物镜的重要原因。
Simultaneous multi-color imaging is a technology commonly used to increase the speed of high content screening systems. However, one of the main problems arising from this approach is spectral crosstalk which can limit assay sensitivity. To overcome this challenge, we have developed an innovative op ...
The Opera Phenix™ high-content screening system’s state of the art hardware, combined with Harmony® imaging and analysis software, improves acquisition and analysis speed, enabling users to perform highly multi-parametric phenotypic screens effectively. In this technical note, learn how the Opera Ph ...
3D cell model imaging and analysis workflows provide more physiologically relevant insights but are not without their challenges. Whether you’re familiar with high-content screening or you want to take your analysis of 3D cell models to the next level, you’ll need the right tools and strategies to o ...
无论是否熟悉高内涵筛选，您均需使用正确的工具和策略来克服在试验中使用复杂3D细胞模型时遇到的难题。例如，培养一致且可再生的3D细胞时会遇到问题，生成厚大细胞样品的图像时会遇到难题，而管理生成的大量数据可能是最困难的部分。本文对成功利用3D细胞模型开展高内涵筛选试验提供了重要建议。通过本文，您可学习如何：生成一致的3D细胞模型、获得最优质的图像、最大化缩短成像时间与数据量、从3D细胞模型中获得更深入的信息与避免不必要的数据传输步骤。
This guide provides instructions on how to find help and manuals for your HCS Hardware/Software (Opera Phenix Plus, Operetta CLS, Harmony).
无论是希望利用高通量筛选探索复杂3D细胞模型的生理相关性，还是希望升级3D细胞模型分析（从简单的酶标仪升级为高内涵），均需要使用正确的工具与策略才能克服模型造成的难题。
Precision oncology aims to match the right treatment to the right patient using specific features of the individual patient's tumor. To increase success, there is a growing advocacy to complement genomics-based approaches with functional drug testing. Here, we provide examples of how the field has a ...
Live-cell imaging, the study of living cells using microscopy, has become a requisite technology in many fields of biomedical research, such as cell biology, developmental biology and cancer research. Also, in drug discovery, researchers adopt live-cell imaging as they look for a more detailed under ...
3D immune cell killing assays yield more physiologically relevant results and are critical to the pursuit of more specialized therapies for the treatment of solid tumors. But they aren't without various technical and logistical difficulties. To address these challenges, this paper presents several s ...
Within diabetes research, an evolving area of interest is the identification and development of an efficacious treatment for ß cell loss. The following literature review discusses the use of a Disque platform for evaluating the functionality of multiple drug candidates.
The incidence and prevalence of non-alcoholic fatty liver disease (NAFLD), which is characterized by excessive accumulation of lipids within the liver, is high and progressively rising due to increasing obesity and metabolic syndrome prevalence. For some patients, NAFLD can progress to non-alcoholic ...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the virus that causes COVID-19, the respiratory illness responsible for the COVID-19 pandemic. Studies have shown that patients infected with SARS-CoV-2 with cardiovascular comorbidities are more susceptible to severe infection and thus ...
With powerful potential for phenotypic drug discovery, cell painting is being rapidly adopted by leading scientists across the globe. However, implementing cell painting assays can present some challenges. To help you avoid some of the common pitfalls, we’ve collated answers from our experts to the ...
This case study shows how a previously-described neuroprotection assay was easily and directly transferred to the Opera Phenix® high-content screening system, with a 4-fold decrease in acquisition time. In the assay, primary rat neurons are co-cultured on top of rat-derived astrocytes. To induce axo ...
This case study illustrates how PerkinElmer’s high-content imaging solutions are enabling therapeutic antibody development. Two steps in the workflow - the clonal B cell selection and the functional antibody characterization - rely on high-content screening using the Operetta® system. The coupling o ...
Antimicrobial resistance (AMR) is one of today’s major global public health challenges. Recently, there has been renewed investment in the discovery of novel antimicrobials to urgently address the growing number of drug-resistant infections. This case study describes how Professor Gordon Dougan and ...
越来越多的研究人员开始研究3D细胞培养、微组织与细胞器，以缩小2D细胞培养与体内动物模型间的差距。本白皮书记录了利用珀金埃尔默提供的解决方案尽快获得最多信息的简化程序。
Researchers are striving to determine where and when relevant bacteria play a role in cancer progression and whether those interactions are localized to the gastrointestinal tract or occur in other tissues before malignancy. Understanding the role bacteria play, whether it be oncogenesis, tumor prom ...
There is a huge unmet need for the development of treatments that target the underlying causes of neurodegenerative diseases. To address the high failure rates and low investment returns of traditional neuroscience R&D approaches, companies are increasingly adopting alternative R&D business models t ...
There has been a lot of buzz around artificial intelligence, machine learning and deep learning. Is the reality living up to the hype? In the world of cellular imaging and its application to drug discovery, there is evidence of real progress against some of the critical challenges facing scientists ...
At the University of Milano-Bicocca, researchers in the laboratory of Professor Francesco Peri have been investigating the impact of molecules produced by microbiota metabolism, known as postbiotics, on the human host. The team has been using using high-content imaging and AlphaLISA immunoassays to ...
Phenotypic Drug Discovery Re-Imagined Cell Painting is a phenotypic screening method and a powerful application of high-content screening technology which combines cell and computational biology to elucidate the behavior of cells under the influence of perturbagens, such as chemical compounds, drugs ...
A team of researchers exploits high-content imaging to phenotype the effects of antimicrobial exposure on individual bacteria cells and screen for novel alternatives to existing antimicrobials. Projects include the development of polyclonal antibody serum, monoclonal antibodies, and functional assay ...
Tumor cell function is critically dependent on the local microenvironment and needs to be considered when devising therapeutic strategies to overcome resistance. In this white paper, we discuss how 3D models will help close the gap between what is seen in the preclinical setting and translation into ...
Today's drug discovery strategies require candidate compounds to fail early and cheaply in the discovery stage, rather than late and expensively in the clinical phase. Testing compounds early in physiologically relevant model systems and leveraging rich information from image-based screens are ways ...
