LC 300 HPLC & UHPLC System | PerkinElmer
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LC 300 UHPLC系统

PerkinElmer LC 300平台重新定义了液相色谱体验,提供了处理最具挑战性分析需求所需的灵活性、性能和效率。我们提供多种检测器和配件,可定制化您的体验,每个LC 300系统都是为满足您最重要的规格而设计的。搭配Simplicity Chrom™ CDS 软件,LC 300 HPLC系统提升了用户体验,包含简化的液相色谱仪工作流程、直观的功能和自动化流程。

货号
型号名称
N2550110
LC 300 HPLC System
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N2550150
LC 300 Analytical HPLC System
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N2550120
LC 300 UHPLC System
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详细信息

The PerkinElmer LC 300 HPLC and UHPLC System has been designed to increase laboratory productivity and throughput, even in the face of challenging budget and staffing situations. Paired with the intuitive SimplicityChrom Chromatography Data System (CDS) Software, our LC 300 HPLC and UHPLC System are the ideal solution for labs seeking to maximize their liquid chromatography experience. The LC 300 HPLC and UHPLC System offers superior performance and flexibility throughout its intelligent design:

  • Robust and precise autosampler with integrated column oven and preheater, as well as a high visibility color LCD display showing full instrument system status, current queue and sample progress.
  • Optional external Peltier column oven for ultra-precise heating and cooling, for sample integrity and exceptionally accurate results.
  • Patented Intermediate Loop Decompression (ILD) valve ensures the injection of an accurate and reproducible sample volume, while also increasing the lifetime of columns used in analysis.
  • Automated, onboard sample dilution and derivatization capabilities simplify the front-end analytical processes, while also ensuring reproducibility and precision.
  • 6k psi analytical pump for reliable and robust performance day after day. 10k psi solvent delivery pump for high-end HPLC functionality, delivering repeatable solvent delivery at a wide range of flow rates and pressures. Powerful 18k psi pump for ultra-precise UHPLC analyses, delivering repeatable solvent delivery at a wide range of flow rates and pressures.
  • Automatic priming design allows the system to achieve full self-priming capabilities, automating the otherwise cumbersome priming process utilized in many other systems.
  • Built-in solvent degassing technology simplifies the analytical process, and obviates the need for a separate, external degassing module.
  • Portfolio of five available detectors, which can be used individually or in sequence.
    • Photodiode array (PDA)
    • Multiwavelength UV/Vis (MWD)
    • Single wavelength UV/Vis
    • Refractive index (RI)
    • Fluorescence (FL)
  • Efficient and powerful instrument control with SimplicityChrom CDS Software, allowing customizable workflows and experiences and supporting compliance with 21 CFR Part 11 requirements.

规格

型号名称 LC 300 UHPLC System

配置

Depth

22 inches

Weight 183 pounds (single stack with one detector and standalone solvent organizer) or 260 pounds maximum (dual stack with external oven)
Width 13.5 inches maximum per component
资源
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应用简报

Qualitative Identification of Tortoise-Shell Glue Using LC/MS/MS

This application note describes a regulatory LC/MS/MS method for the qualitative identification of tortoise-shell glue, an important Traditional Chinese Medicine (TCM) known for its hemopoietic and hemostatic properties.

应用简报
Oleanlic Acid and Ursonic Acid Analysis of Chaenomelis Fructus by LC-UV

This application note outlines an LC-UV method for analyzing oleanolic acid and ursonic acid in Chaenomelis fructus, known for its medicinal properties.

应用简报
Workflow for Qualitative Identification of Donkey-Hide Gelatin

This application note discusses a qualitative LC/MS/MS workflow for identifying donkey-hide gelatin, a valuable traditional Chinese medicinal product.

应用简报

应用文献

Glutaraldehyde Analysis in Bioprosthetic Heart Valve by LC-UV

This application note presents a detailed LC-UV workflow for analyzing glutaraldehyde in bioprosthetic heart valves using the PerkinElmer LC 300 system.

应用文献
Paeoniflorin Analysis in Paeoniae Radix Alba by LC-UV

This application note describes the quantification of paeoniflorin in Paeoniae radix alba using a PerkinElmer LC 300 UHPLC system. By employing a polar column, the method enhances separation performance and meets the Chinese Pharmacopoeia specifications.

应用文献
Rapid Analysis of Gelatinous Traditional Chinese Medicine

This document details a rapid LC/MS/MS workflow for analyzing gelatinous traditional Chinese medicines using the PerkinElmer QSight LX50 UHPLC system.

应用文献
Analysis of Aflatoxins in Traditional Chinese Medicine Coix Seed Using LC/MS/MS

This application note presents a sensitive LC/MS/MS method for detecting aflatoxins in Coix seed, a traditional Chinese medicine. The analysis, conducted on a PerkinElmer LC 300 system, meets the stringent sensitivity and accuracy requirements of the Chi

应用文献
Characteristic Components Analysis in Lonicerae Japonica Flos by LC-UV

This application brief details a comprehensive LC-UV method for analyzing multiple characteristic components in Lonicerae japonica flos, used for treating inflammation and infectious diseases.

应用文献
Chlorogenic Acid Analysis in Children Throat-Reliever Granule by LC-UV

This application note presents an LC-UV workflow for the quantification of chlorogenic acid in children's throat-reliever granules, a traditional Chinese medicine. The analysis was conducted using a PerkinElmer LC 300 UHPLC system with a C18 column.

应用文献
HPLC Analysis of Oleanolic Acid and Ursonic Acid in Eriobotrya Japonica Leaf

This document outlines a robust LC-UV method for quantifying the triterpene acids oleanolic acid and ursonic acid in Eriobotrya japonica leaf, a traditional Chinese medicinal material. Utilizing a PerkinElmer LC 300 system with a C18 column, the method a

应用文献
Ferulic Acid Analysis in Angelica Sinensis Radix by HPLC

This application note describes a detailed LC-UV workflow for analyzing ferulic acid, an important phenolic acid used for quality assessment, in Angelica sinensis (A. sinensis). The analysis was performed using a PerkinElmer LC 300 system equipped with a

应用文献

文章

Best Practices for HPLC to UHPLC Method Transfer

Best Practices for HPLC to UHPLC Method Transfer Converting a method from HPLC to UHPLC has a number of benefits, as outlined in the first 2 articles in our series, including faster analytical separations, better peak resolution and lower long-term costs

文章

产品手册

Advanced Solutions for Biofuels Development and Testing

Download this interactive brochure to see all our biofuel lab solutions and supporting services. Maximize your laboratory's prospects in biofuels production and testing with PerkinElmer’s analytical solutions that can help you achieve high product qualit

产品手册

Video

Performance and Productivity with the LC 300 HPLC & UHPLC Systems

For laboratories focused on achieving high levels of efficiency, without compromise on productivity, the PerkinElmer LC 300 HPLC and UHPLC System offers performance and productivity even for the most challenging analytical demands. Discover more.

Video
HPLC Columns: The Foundation of Great Chromatography

The foundation of great Chromatography starts with selecting the right column. Our LC column portfolio brings you new opportunities for full performance-boosting LC workflow solutions, ensuring that you have the optimum column for your application.

Video

网络研讨会

网络研讨会
An Introduction to LC-MS/MS

This presentation is the first in a series that will introduce you to the highly useful analytical technique - High Performance Liquid Chromatography - Mass Spectrometry (LC-MS).

网络研讨会
网络研讨会
Chromatography Simplified – Improving LC Workflow Efficiency and Productivity

Watch our on-demand webinar to discover the difference that a focus on improving the user experience will have on your workflows and productivity.

网络研讨会

产品手册

LC 300 HPLC and UHPLC Systems Interactive Brochure

For laboratories looking to achieve the highest levels of versatility without compromising on productivity, the LC 300 HPLC and UHPLC Systems deliver the flexibility, performance, and efficiency you need for even the most challenging analytical demands. With fully customizable configurations and acc ...

PDF 2 MB
Chromatography Solutions for Soft Drink Analysis

Monitoring the consistency and quality in soft drinks is essential to ensure a positive brand image and a loyal customer base. Our full portfolio of chromatography solutions, including LC, GC and GC/MS, enables you to meet – and even exceed – your quality control goals with rapid, intuitive, and acc ...

PDF 1 MB

产品说明

LC 300 HPLC and UHPLC Systems - Product Note - Redefining LC Versatility, Maximizing Lab Productivity

For laboratories looking to achieve the highest levels of versatility without compromising on productivity, the LC 300 HPLC and UHPLC Systems deliver the flexibility, performance, and efficiency you need for even the most challenging analytical demands. With fully customizable configurations and acc ...

PDF 785 KB

单页

SimplicityChrom CDS

SimplicityChrom™ is a modern, intuitive and flexible CDS software platform that enables users to efficiently control their instrument, process data, and generate results. Pair this functionality with the peace of mind of 21 CFR Part 11 compliance and industry-leading service and support, and you’re ...

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Semiconductor & Electronics Analysis Overview Flyer

Download this Flyer for an overview of PerkinElmer's analytical solutions for the Semiconductor industry. This 2-page flyer outlines semiconductor and microelectronic workflows and applications, and the precise and reliable PerkinElmer solutions to address them - including instrumentation, consumabl ...

PDF 1 MB

应用文献

通过HILIC结合示差折光检测器分析枫糖浆中的糖分

枫糖浆是一种常见的天然甜味剂,世界各地家庭都经常食用。枫糖浆中糖分含量的量化对于满足产品标签要求和确保标签声明的准确性至关重要。虽然蔗糖是枫糖浆中的主要糖分,但对其他复杂碳水化合物(包括葡萄糖、果糖和麦芽糖)进行完整的鉴定和量化是必不可少的。在本应用说明中,提出了一种测定上述四种复合碳水化合物的有效方法。采用LC 300 HPLC系统,并利用亲水性相互作用色谱(HILIC)技术,随后通过LC 300示差折光(RI)检测器实现检测。

PDF 1 MB
HPLC/UV法分析膳食补充剂中的植物雌激素异黄酮

异黄酮是一种水溶性化合物,存在于许多植物和食物中。异黄酮被命名为植物雌激素,在结构上与雌性激素相似,并已被证明对人类健康有许多有益的影响,例如减少某些癌症的发生,以及改善胆固醇比率。然而,研究也表明,异黄酮的植物雌激素性质也会导致现有甲状腺疾病的恶化,因为内分泌系统受到干扰。因此,精确定量保健品中的异黄酮含量是至关重要的,以确保标签声明的准确性和消费者的安全。在本应用说明中,阐述了一种高效、可靠的测定六种常见异黄酮化合物的方法。该方法是根据美国药典关于膳食补充剂胶囊中大豆异黄酮的专著而开发的,采用PerkinElmer LC 300 UHPLC系统分离,并通过LC 300 PDA检测器检测化合 ...

PDF 1 MB
采用带有PDA检测器的PerkinElmer LC 300 HPLC系统分析16种大麻素

准确定性和定量分析大麻素是将安全和高质量大麻花和强化产品推向市场的关键步骤。随着世界各地娱乐性大麻使用持续合法化,需要快速有效的液相色谱法测定大麻素含量,以满足监管和消费者的需求。本文介绍了16种常见大麻素的快速简便测定方法。利用带PDA检测器的PerkinElmer LC 300 HPLC系统,该方法可在7分钟内洗脱所有16种化合物,大多数分析物的定量限为0.15µg/mL。

PDF 1 MB
通过HPLC分析牛奶和奶酪制品中的乳糖

乳糖是乳制品中的主要碳水化合物。在全球范围内,乳糖的分析尤其重要,因为许多人患有乳糖不耐受症。本项工作阐述了一种常规样品制备和定量分析马苏里拉乳清干酪和牛奶中乳糖的方法。由于乳糖可以水解或酶解产生单糖类葡萄糖和半乳糖,这两种糖可以色谱分离,但不能定量。这个方法使用带示差折光(RI)检测器的Altus™ HPLC 系统,特别适用于分析很少或没有紫外线吸收的分析物,如许多食糖。

PDF 1 MB
HPLC: Analysis of Acrylate Monomers

Acrylate polymers are utilized for a host of applications due to their varied physical properties and ease of synthesis. These acrylates are sought after specifically for coatings, adhesives, sealants and elastomers (CASE) applications because of their stability, high heat resistance, excellent weat ...

PDF 1 MB
HPLC: Rapid Quantitation of Vitamins in Biological Fluids using HPLC and IVD-CE Certified Kits

Vitamins are involved in a variety of different physiological processes and must be assimilated with the daily nutrition as they cannot be synthesized within the human body. The biochemical functions of vitamin C (ascorbic acid) are mainly linked to its antioxidant and radical scavenger properties. ...

PDF 597 KB
Analysis of Common Preservatives in Personal Care Products by HPLC with UV Detection

Parabens are utilized as an antimicrobial preservative in many commercial offerings including cosmetics, personal care products and foods, and offer both chemical and thermal stability to products. Although parabens increase the safety of products, recent studies have suggested that parabens may exh ...

PDF 1 MB
HPLC: Analysis of mRNA with PDA Detection

The recent emergence of mRNA-based vaccines and therapies has made clear the need for reliable analytical methods that can successfully identify, characterize, and quantify mRNA. This chemical instability demands a deeper understanding of the mechanisms by which mRNA degrades, which can inform the d ...

PDF 816 KB
Analysis of Saccharides in High Fructose Corn Syrup

High fructose corn syrup (HFCS) as a substitute for sucrose in a wide variety of products, including baked goods, sauces, beverages, canned fruits and dairy products. HFCS is produced from regular corn syrup, with an enzymatic process that isomerizes some of the corn syrup's glucose into fructose. T ...

PDF 1 MB

应用简报

HPLC Analysis of Lamotrigine Using LC 300 in Accordance With USP

Lamotrigine is an antiepileptic drug in the phenyl triazine class used in the treatment of both epilepsy and as a mood stabilizer for bipolar disorder. It is the first medication since lithium to be granted US FDA approval for maintenance treatment of bipolar type 1, and is approved for use in more ...

PDF 370 KB
HPLC Analysis of Triamcinolone Acetonide Purity Using LC 300 in Accordance With USP

This Application Brief demonstrates the ability of PerkinElmer's LC 300 instrument platform, and SimplicityChrom™ chromatographic data system (CDS) software to successfully meet the United States Pharmacopeia (USP) performance requirements for the HPLC analysis of Triamcinolone Acetonide purity. ...

PDF 322 KB
HPLC Assay of Triamcinolone Acetonide Using LC 300 in Accordance With USP

This Application Brief demonstrates the ability of PerkinElmer's LC 300 instrument platform, and SimplicityChrom™ chromatographic data system (CDS) software to successfully meet the United States Pharmacopeia (USP) performance requirements for the HPLC analysis of Triamcinolone Acetonide. ...

PDF 353 KB
HPLC Assay of Risperidone Impurities Using LC 300 in Accordance With USP

This Application Brief demonstrates the ability of PerkinElmer's LC 300 instrument platform, and SimplicityChrom™ chromatographic data system (CDS) software to successfully meet the United States Pharmacopeia (USP) performance requirements for the HPLC analysis of Risperidone impurities. ...

PDF 316 KB
HPLC Assay of Risperidone Using LC 300 in Accordance With USP

Risperidone is an antipsychotic medicine that works by changing the effects of chemicals in the brain. The methods for assessing Risperidone characteristics are described in the United States Pharmacopeia and rely on chromatographic techniques. In this application brief, we demonstrate the suitabili ...

PDF 285 KB
HPLC Analysis of Omeprazole Using LC 300 in Accordance with USP

The World Health Organization regards omeprazole among its ‘List of Essential Medicines’ which outlines the most effective and safest medicines required by a country's healthcare system to meet its needs. The United States Pharmacopeia (USP) closely monitors the production of these drugs and provide ...

PDF 274 KB
HPLC Analysis of Pregabalin Using LC 300 in Accordance With USP

Pregabalin is an anti-epileptic drug that works by slowing down impulses in the brain that cause seizures. It can also be utilized to treat fibromyalgia, or nerve pain in people with diabetes, herpes zoster, or spinal cord injury owing to its ability to affect chemicals in the brain that send pain s ...

PDF 292 KB

技术说明

珀金埃尔默LC 300 自动进样器中心回路泄压(ILD)进样阀

向UHPLC系统进样准确且可重复的样品体积并非易事,但对于确保高质量分析至关重要。LC 300自动进样器中配置的珀金埃尔默专利中心回路卸压(ILD)阀是一个转子密封处专门放置径向凹槽的7通阀,可确保准确和可重复样品体积的进样,同时也增加了分析中使用的色谱柱寿命。

PDF 849 KB

指南

How to Guide for HPLC: Choosing the Right Detector

There are many characteristics to consider when choosing a detector, therefore several detectors have been developed over time to address particular challenges. It is important to consider the chemical nature of the analytes of interest and any potential interferences, when choosing a detector. Addi ...

PDF 766 KB