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LC 300 UHPLC System

The PerkinElmer LC 300 platform redefines the liquid chromatography experience, offering the flexibility, performance and efficiency needed to tackle even the most challenging analytical demands. With the option to customize your experience with a wide range of detectors and accessories, each LC 300 system is designed to meet the most important specifications – yours. Paired with SimplicityChrom CDS software, the LC 300 UHPLC system delivers an elevated user experience with simplified liquid chromatography workflows, intuitive functions, and automated processes.

Part Number N2550120
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The PerkinElmer LC 300 UHPLC system has been designed to increase laboratory productivity and throughput, even in the face of challenging budget and staffing situations. Paired with the highly intuitive and powerful SimplicityChrom chromatography data system (CDS) software, our LC 300 systems are the ideal solution for labs seeking to maximize their liquid chromatography experience.

The LC 300 offers superior performance and flexibility throughout its intelligent design:

  • Robust and precise autosampler with integrated column oven and preheater, as well as a high visibility color LCD display showing full instrument system status, current queue and sample progress.
  • Optional external Peltier column oven for ultra-precise heating and cooling, for sample integrity and exceptionally accurate results.
  • Patented Intermediate Loop Decompression (ILD™) valve ensures the injection of an accurate and reproducible sample volume, while also increasing the lifetime of columns used in analysis.
  • Automated, onboard sample dilution and derivatization capabilities simplify the front-end analytical processes, while also ensuring reproducibility and precision.
  • Powerful 18k psi pump for ultra-precise UHPLC analyses, delivering repeatable solvent delivery at a wide range of flow rates and pressures.
  • Automatic priming design allows the system to achieve full self-priming capabilities, automating the otherwise cumbersome priming process utilized in many other systems.
  • Built-in solvent degassing technology simplifies the analytical process, and obviates the need for a separate, external degassing module.
  • Portfolio of five available detectors, which can be used individually or in sequence.
    • Photodiode array (PDA)
    • Multiwavelength UV/Vis (MWD)
    • Single wavelength UV/Vis
    • Refractive index (RI)
    • Fluorescence (FL)
  • Efficient and powerful instrument control with SimplicityChrom CDS, allowing users to create custom workflows and experiences, all while achieving compliance with 21 CFR Part 11 requirements.


Depth 22 inches
Height 38.2 inches maximum (single stack with detector and standalone solvent organizer)
Weight 183 pounds (single stack with one detector and standalone solvent organizer) or 260 pounds maximum (dual stack with external oven)
Width 13.5 inches maximum per component
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Application Brief

Characteristic Components Analysis in Lonicerae Japonica Flos by LC-UV

Lonicera Japonica Flos is a Traditional Chinese Medicine (TCM) used for the treatment of inflammation and infectious diseases. Chinese Pharmacopoeia has developed a method to support quality control efforts which includes the analysis of seven components in Lonicera Japonica Flos samples.

I ...

Chlorogenic Acid Analysis in Children Throat-Reliever Granule by LC-UV

Utilized for throat inflammation in children, Xiaoer Yanbian Keli granules are a traditional Chinese formulated medicine, comprised of the Lonicera Japonica herbal plant. Chinese Pharmacopoeia developed standards in 2020 to ensure quality control, and has finalized the assay of a method in which ...

PDF 754 KB
HPLC Analysis of Acetaminophen, Aspirin and Caffeine Using an Epic C18 Column in Accordance with the USP

Acetaminophen, aspirin and caffeine used in combination are an effective method of treating acute headaches and migraines. The combination of these substances is considered superior to acetaminophen on its own when treating headaches. There are other benefits due to the individual components of t ...

PDF 598 KB
HPLC Analysis of Amlodipine Besylate in Accordance with the USP

Amlodipine besylate belongs to a class of drugs known as dihydropyridine calcium channel blockers. It is primarily used to treat hypertension and angina as well as other conditions which arise as a result of coronary artery disease. As a calcium channel blocker, it prevents diffusion of calcium i ...

PDF 719 KB
HPLC Analysis of Betamethasone Dipropionate Using Epic C18 HPLC Column

Glucocorticoid steroids work by suppressing various aspects of the human immune system in conditions where hyperactivity can cause poor health through allergies, inflammation, and autoimmune dysfunction. Betamethasone dipropionate belongs to this class of steroids. It is used for its high potency ...

PDF 741 KB
HPLC Analysis of Common Ginsenosides Found in Ginseng Using the Chinese Pharmacopeia

The term ginseng can refer to several natural products and herbal remedies usually produced from the plant, panax ginseng. Panax ginseng is considered a traditional Chinese medicine (TCM) as it has a reported wide range of medicinal properties such as antioxidant effects and promotion of immune f ...

PDF 959 KB
HPLC Analysis of Finasteride Using an Epic C18 HPLC Column

Finasteride is an antiandrogenic compound or ‘testosterone blocker’ which is used as a treatment for an enlarged prostate and can treat male hair loss. It inhibits the production of the enzyme Type-II 5a-reductase which converts testosterone into the more active metabolite 5a-dihydrotestosterone ...

PDF 689 KB
HPLC Analysis of Hydrocortisone in Accordance with the United States Pharmacopeia

Hydrocortisone is the pharmaceutical term for cortisol used in oral administration, intravenous injection, or topical application. It is often used in the treatment of severe allergic reactions, such as anaphylaxis and angioedema. United States Pharmacopeia (USP) provides monographs for the ident ...

PDF 650 KB
HPLC Analysis of Lamotrigine Related Compound B Impurity in Accordance with the USP

Lamotrigine is an antiepileptic drug belonging in the phenyl triazine class. It is used in the treatment of both epilepsy and as a mood stabilizer for bipolar disorder. Lamotrigine related compound B also known as 2,3-dichlorobenzoic acid is a key intermediate for the antiepileptic drug Lamotrigi ...

PDF 407 KB
HPLC Analysis of the Semi-Synthetic Antibiotic Azithromycin Using an Epic C18 Column

Azithromycin, discovered in 1980, is a semi-synthetic macrolide antibiotic related to erythromycin. The drug is widely used to treat chest infections such as pneumonia, infections of the nose and throat such as sinus infection (sinusitis), skin infections, Lyme disease, and some sexually transmit ...

PDF 302 KB
Oleanlic Acid and Ursonic Acid Analysis of Chaenomelis Fructus by LC-UV

Chaenomeles, the fruit of the Chinese flowering quince, is utilized in Traditional Chinese Medicine (TCM) for its analgesic, anti-inflammatory, antispasmodic and astringent properties, and has also been shown to aid in digestion. For implementation of quality control processes, Chinese Pharmacopo ...


Application Note

Analysis of 16 Cannabinoids Using the PerkinElmer LC 300 HPLC System with PDA Detection

Accurate identification and quantification of cannabinoid content is a critical step in the process to bringing safe and high quality cannabis flower and fortified products to market. As the legalization of recreational cannabis use continues throughout the world, fast and efficient liquid chroma ...

Analysis of Common Preservatives in Personal Care Products by HPLC with UV Detection

Parabens are utilized as an antimicrobial preservative in many commercial offerings including cosmetics, personal care products and foods, and offer both chemical and thermal stability to products. Although parabens increase the safety of products, recent studies have suggested that parabens may ...

Analysis of Phytoestrogen Isoflavones in Dietary Supplements by HPLC/UV

Isoflavones are water-soluble compounds found in many plant and food sources. Designated as phytoestrogens, isoflavones are structurally similar to to the female hormone estrogen, and have been shown to exhibit a number of beneficial impacts to human health, such as a reduction in the developmen ...

Analysis of Saccharides in High Fructose Corn Syrup

High fructose corn syrup (HFCS) as a substitute for sucrose in a wide variety of products, including baked goods, sauces, beverages, canned fruits and dairy products. HFCS is produced from regular corn syrup, with an enzymatic process that isomerizes some of the corn syrup's glucose into fructose ...

Analysis of Sugars in Maple Syrup by HILIC with Refractive Index Detection

Maple syrup, a common natural sweetener, is consumed regularly by households around the world. Quantification of sugar content in maple syrup is paramount to satisfy product labeling requirements and ensure label-claim accuracy. Although sucrose is the primary sugar component in maple syrup, a co ...

HPLC Analysis of Lamotrigine Using an Epic C18 SD Column in Accordance with the United States Pharmacopeia

Lamotrigine is an antiepileptic drug in the phenyl triazine class used in the treatment of both epilepsy and as a mood stabilizer for bipolar disorder. It is the first medication since lithium to be granted US FDA approval for maintenance treatment of bipolar type 1, and is approved for use in mo ...

PDF 745 KB
HPLC Analysis of Pregabalin Using Epic C18 Column in Accordance With USP

Pregabalin is an anti-epileptic drug that works by slowing down impulses in the brain that cause seizures. It can also be utilized to treat fibromyalgia, or nerve pain in people with diabetes, herpes zoster, or spinal cord injury owing to its ability to affect chemicals in the brain that send pai ...

PDF 466 KB
Paeoniflorin Analysis in Paeoniae Radix Alba by LC-UV

Paeoniae radix alba, also known by its Chinese phonetic name as Baishao, is a Traditional Chinese Medicine herbal plant used to treat inflammatory disorders. Paeoniflorin, the major active component extracted from Paeoniae radix alba, offers proven efficacy against gynecological disorders, pain, ...



Chromatography Solutions for Soft Drink Analysis

Monitoring the consistency and quality in soft drinks is essential to ensure a positive brand image and a loyal customer base. Our full portfolio of chromatography solutions, including LC, GC and GC/MS, enables you to meet – and even exceed – your quality control goals with rapid, intuitive, and ...

Comprehensive Testing Solutions for Feed Millers

Feed milling today is a complex business, and agribusinesses both large and small need to balance the nutritional and safety needs of livestock with availability of raw ingredients and their seasonality and variability. Add to that the valuable supplementation and medication that millers supply, ...

LC 300 HPLC and UHPLC Systems Interactive Brochure

Laboratories in nearly every industry are continuously faced with growing demands for increased throughput, improved margins, and more efficient processes. And when it comes to liquid chromatography, a one-size-fits-all approach just isn’t enough. You need an LC system tailored to your needs, wit ...



Epic HPLC-UHPLC Column Phase Specifications Sheet

The Epic™ line is our latest range of LC columns, based on high density monomerically bonded phases produced through a proprietary bonding process. Epic HPLC and UHPLC columns are compatible with a wide range of organic modifiers and buffers and stable over a wide pH range. All Epic products use ...

PDF 294 KB
SimplicityChrom CDS

SimplicityChrom is a modern, intuitive and flexible CDS software platform that enables users to efficiently control their instrument, process data, and generate results. Pair this functionality with the peace of mind of 21 CFR Part 11 compliance and industry-leading service and ...



How to Select the Right HPLC Columns

Selecting the right HPLC Column for your analysis can make all the difference. There are many considerations when selecting columns, like the molecular weight of your target analytes, their solubility characteristics, and their interactions with stationary phase chemistries.

Download our Se ...

PDF 457 KB

Product Note

LC 300 Product Note

The PerkinElmer LC 300 liquid chromatography systems are the result of listening to user needs, and paying careful attention to every detail in the resulting design. The LC 300 systems are the ideal solution for labs faced with high throughput requirements or challenging chromatographic separatio ...

PDF 950 KB

Technical Note

A Comparison of Fully Porous C18 Reversed Phase HPLC Columns

The most commonly used LC reversed-phase alkyl bonded stationary phase is octadecyl carbon chain (C18)-bonded silica, which is denoted as USP classification L1. C18 columns have a broad applicability from pharmaceuticals to food and environmental analyses. However, not all C18 columns are alike. ...

Clone Columns Aviator C18 and ACE C18

Legacy methods, by their very nature, often use older column technologies. These older phases can be accompanied by larger variations in batch-to-batch performance which can lead to inconsistent results and cause out of specification (OOS) occurrences. With routine analysis, often completed with ...

PDF 835 KB
Clone Columns Sonoma C18(2) and Luna C18(2)

Legacy methods, by their very nature, often use older column technologies. These older phases can be accompanied by larger variations in batch-to-batch performance which can lead to inconsistent results and cause out of specification (OOS) occurrences. With routine analysis, often completed with ...

PDF 790 KB
Clone Columns-Harmony C18 and Waters Symmetry C18 Comparison

Legacy methods, by their very nature, often use older column technologies. These older phases can be accompanied by larger variations in batch-to-batch performance which can lead to inconsistent results and cause out of specification (OOS) occurrences. With routine analysis, often completed with ...

PDF 881 KB
PerkinElmer LC 300 Autosampler Intermediate Loop Decompression (ILD) Injection Valve

The injection of an accurate and reproducible sample volume into a UHPLC system is challenging, but essential to ensuring a high quality analysis. The patented PerkinElmer Intermediate Loop Decompression (ILD) valve, featured in the LC 300 Autosampler, is a 7-port valve with a strategically place ...

PDF 849 KB