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| 3D Volumetric Analysis of Luminal Spaces Inside Cysts or Organoids High-content assays using 3D objects such as cysts or organoids can be challenging from the perspectives of both image acquisition and image analysis. In this technical note, we describe how to image and analyze epithelial Madin-Darby canine kidney (MDCK) cysts in 3D on the Operetta CLS™ high-content analysis system. Equipped with water immersion objectives and Harmony® 4.8 high-content imaging and analysis software, the Operetta CLS is able to overcome the most critical of challenges with 3D assays and provides an ideal package for 3D imaging and 3D phenotypic characterization of more physiologically relevant cell culture models. |
| How to Perform Successful Long Term Live Cell Imaging in a High-Content Analysis System Live cell imaging has gained importance within drug discovery over recent times, as researchers look for more meaningful insights into cellular behavior and function. In this technical note, we monitor evaporation rates, cell growth and morphology inside the temperature- and CO2-controlled live cell chamber of the Operetta CLS™ high-content analysis system, and compare the results with those obtained using a humidity-controlled cell culture incubator. Download our technical note to find out how you can overcome some of the challenges associated with long-term live cell imaging. |
| Distinguishing Cell Types by Phenotypic Profiling of the Nucleus The promise of high-content screening is the acceleration of discovery by extracting as much relevant information as possible from cells. Nevertheless, a large percentage of high-content screens analyze only a small number of image-based properties. In this application note, we show how a single nuclear stain can enable phenotypic profiling and how phenotypic profiles can be used to distinguish up to seven different cell types, without further staining or phenotypic markers. |
| The Benefits of Automated Water Immersion Lenses for High-Content Screening Balancing the key factors in HCS imaging - sensitivity, resolution and speed - can be challenging since they cannot be optimized independently: changing one impacts the others. Nevertheless, there is a way to overcome some of the obstacles and here we explain why the choice of the objective lens is critical. |
For Research Use Only. Not for Use in Diagnostic Procedures.
